Fluorescence labeling biological material and preparation method thereof

Abstract

The invention discloses a fluorescence labeling biological material and a preparation method thereof. The method comprises the following steps: (1) activating fluorescent molecules; (2) carrying out areaction on a polymer and activated fluorescent molecules; (3) dialyzing the solution after the reaction in the step (2) with deionized water, and freeze-drying to obtain a fluorescence labeling biological material, wherein the polymer is a polyamino acid-based polymer or a chitosan-based polymer. According to the invention, the carboxyl on a fluorescent molecule is activated into active ester, then the active ester and the amino on a biopolymer are subjected to an amidation reaction, and the fluorescent molecule is chemically coupled to the biopolymer, so that the fluorescence labeling material has different fluorescence colors due to different labeled fluorescence molecules, and the chemical labeling method avoids the defect that fluorescent molecules or particles thereof are easy to diffuse out of a system in physical mixing or electrostatic adsorption and other methods; and the fluorescence labeling biological material has a wide application prospect in the research fields of in-vitro and in-vivo imaging, tracing, material degradation, biosensing, 3D printing and the like.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a fluorescent-labeled biological material and a preparation method thereof. Background technique [0002] Since the concept of tissue engineering was put forward, people have developed many types of biomaterials, some of which are polyamino acid materials such as collagen, gelatin and polylysine, etc., and polysaccharide materials such as chitosan are rich in amino groups in their molecular structures. It not only provides positively charged adhesion sites for cell adhesion and proliferation, but also provides active groups for chemical modification. [0003] Fluorescent probes have important applications in in vivo and in vitro imaging, material tracking, biosensing and other fields. Currently commercially available fluorescent probes are mostly quantum dots and polymer microspheres. Combining them with biological materials has a good fluorescent effect...

AI Technical Summary

Problems solved by technology

Fluorescent particles are mostly stored in emulsion, use more organic solvents, and have high biological toxicity after adding materials

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