Preparation method and application of selaginella fermentation extract
A technology for extracting liquid and Selaginella, which is applied in the field of probiotic fermentation of Selaginella to prepare extract and high activity lysate, can solve the problem of limited nutrients in Selaginella, achieve enhanced moisturizing, achieve green and environmental protection, good low immunity original effect
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Embodiment 1
[0029] A preparation method of Selaginella fermented extract:
[0030] 1) Activation of fermentation strains
[0031] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.
[0032] 2) Preparation of fermentation medium
[0033] Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried Selaginella leaves and pulverize to obtain 60-mesh Selaginella powder. Add 0.1wt% Selaginella powder, 0.1wt% glucose, 0.001wt% casein, 0.001wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.
[0034] 3) ...
Embodiment 2
[0039] A preparation method of Selaginella fermented extract:
[0040] 1) Activation of fermentation strains
[0041] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.
[0042] 2) Preparation of fermentation medium
[0043]Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried leaves of Selaginella and pulverize to obtain 80 mesh Selaginella powder. Add 1wt% Selaginella powder, 1wt% glucose, 0.02wt% casein, 0.005wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.
[0044] 3) Fer...
Embodiment 3
[0049] A preparation method of Selaginella fermented extract:
[0050] 1) Activation of fermentation strains
[0051] Weigh 0.6g of sodium acetate, 4g of soybean peptone, 4g of glucose, 1g of yeast extract, 0.4g of dipotassium hydrogen phosphate, 0.12g of magnesium sulfate, 0.05g of manganese sulfate, and 200ml of distilled water. The bacteria were cooled to room temperature after 15 minutes, and then inserted into the preserved strain Lactobacillus Brucella CGMCC No.19125 for activation and culture for 48 hours to obtain an activated seed medium.
[0052] 2) Preparation of fermentation medium
[0053] Take the whole plant of Selaginella after drying and dehydration, wash and dry, collect the dried leaves of Selaginella and pulverize to obtain 100 mesh Selaginella powder. Add 3wt% Selaginella powder, 2wt% glucose, 0.04wt% casein, 0.01wt% vitamin C to the fermentation system, make up the rest with water, shake well and then sterilize at 121° C. for 15 minutes.
[0054] 3) Fe...
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