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Ratiometric fluorescence sensing method for visually identifying multiple organic solvents

An organic solvent, ratiometric fluorescence technology, applied in the field of ratiometric fluorescence sensing, to achieve the effect of large fluorescence emission wavelength difference, multiple fluorescence colors, and high visual distinction.

Active Publication Date: 2020-05-08
MINNAN NORMAL UNIV
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Problems solved by technology

[0005] The invention provides a ratiometric fluorescent sensing method for visually identifying various organic solvents to solve the problem of how to identify several common organic solvents in a portable manner

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  • Ratiometric fluorescence sensing method for visually identifying multiple organic solvents
  • Ratiometric fluorescence sensing method for visually identifying multiple organic solvents

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Embodiment Construction

[0022] In order to facilitate a better understanding of the present invention, the following examples are used to illustrate, and these examples belong to the protection scope of the present invention, but do not limit the protection scope of the present invention.

[0023] A ratiometric fluorescent sensing method for visually identifying multiple organic solvents, comprising the following steps:

[0024] (1) Preparation of carbon dots (CDs). Weigh 0.5g of citric acid and 0.5g of polyethyleneimine, dissolve in 20mL of ultrapure water, shake and mix well, and react in a hydrothermal reaction kettle at 180°C for 6h, and dialyze the obtained solution through a dialysis bag (molecular weight: 1000) , freeze-dried and redispersed with ultrapure water to obtain a 1 mg / mL stock solution of carbon dots.

[0025] (2) Preparation of gold clusters (AuNCs@BSA). Prepare 10mM chloroauric acid and 5mL of 50mg / mL BSA (bovine serum albumin) solution; stir and mix the chloroauric acid solutio...

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Abstract

The invention discloses a ratiometric fluorescence sensing method for visually identifying multiple organic solvents. The ratiometric fluorescence sensing method comprises the following steps: (1) preparing carbon dots; (2) preparing a gold cluster; (3) modulating the proportion of the fluorescent probe and carrying out liquid phase detection; and (4) performing preparation and visual detection ofthe fluorescent test paper. Fluorescent carbon dots and gold clusters are used as double fluorescent probes to construct a multicolor ratio fluorescence sensing mode. Because different types of organic solvents change different fluorescence intensities of the two probes, the fluorescence of the test paper can present different colors with large difference and is easy to distinguish by naked eyes,thereby realizing visual identification and detection of five organic solvents, namely methanol, acetonitrile, acetone, ethyl acetate and tetrahydrofuran.

Description

【Technical field】 [0001] The invention belongs to the technical field of ratio fluorescence sensing, in particular to a ratio fluorescence sensing method for visually identifying various organic solvents. 【Background technique】 [0002] Whether in industrial production or in the laboratory, the use of organic solvents is indispensable. However, these common organic solvents are usually colorless and transparent liquids, and it is difficult to effectively distinguish them only by senses. Once the label is lost, it will cause difficulties in disposal and waste of resources. If the identification of its components also requires special equipment such as gas chromatography, it is time-consuming and laborious, and the cost is high. For this reason, it is necessary to develop a detection technique for portable identification of common organic solvents. [0003] Fluorescence spectroscopy has the characteristics of high sensitivity, fast response, simplicity and economy. In portab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/77G01N21/78
CPCG01N21/64G01N21/643G01N21/6428G01N21/6447G01N21/6456G01N21/77G01N21/78G01N2021/6495G01N2021/6421G01N2021/7759G01N2021/6439G01N2021/7786Y02A50/30
Inventor 杨伟强张桂云李飞明倪建聪
Owner MINNAN NORMAL UNIV
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