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Immune cell culture method

A culture method and cell culture technology, applied in the field of immune cell culture, can solve the problems of bacterial infection risk, growth factor degradation at 37°C, and high cost

Pending Publication Date: 2020-05-08
SHANGHAI HUAYAN MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Immune cells are now widely used in clinics. Traditional immune cell culture takes about 10 days to carry out a bacterial test, which does not meet the requirements of the Pharmacopoeia, and there is a risk of bacterial infection every time the bacterial test is performed, and the cost is high. At the same time, there are still Degradation of Growth Factors at 37°C

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take the production of cik immune cells as an example

[0042] Step 1. Sample processing - GMP thousand-level laboratory operation:

[0043] 1.1 The peripheral blood is collected as a sample blood sample, and the sample blood sample is first centrifuged (2500rpm, 15min) in the first step to separate plasma and cells.

[0044] 1.2 Extinguish the plasma centrifuged in step 1.1 (56°C, 30min).

[0045] 1.3 The cells in step 1.1 were separated by ficoll method, and monocytes were taken.

[0046] 1.4 Wash the monocytes separated in 1.3 twice (1400rpm, 6min), and set aside.

[0047] 1.5 Centrifuge the inactivated plasma in 1.2 to remove impurities (3000rpm, 6min), and set aside.

[0048] The main reagents used are shown in Table 1:

[0049] Table 1 Reagents used in experiments

[0050]

[0051]

[0052] Step 2. Cultivate immune cells:

[0053] 2.1 Take 10 million monocytes obtained in 1.4 and resuspend them in 10 mL of basal medium (adjust the density to 2 million ...

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Abstract

The invention provides an immune cell culture method, belonging to the technical field of immune cell culture. According to the method, immune cells are transferred into a cell culture bag made of anFEP material from the 4th day of culture; the cell culture bag is put into a cell culture box with a temperature of 37 DEG C; the cell culture bag is connected with a culture medium storage bag filledwith a cell culture medium by using a communicating pipe; and the culture medium storage bag is put into a refrigerator with a temperature of 4 DEG C. When the method disclosed by the invention is used for culturing immune cells, closed culture is realized from the procedure of pre-culture in the culture process enters, so bacterial pollution can be avoided; the complete medium is preserved at 4DEG C, so a factor titer is stable, and degradation of nutrient substances at 37 DEG C is avoided; and sterile detection of cell products is more convenient and faster, and cost is low.

Description

technical field [0001] The invention belongs to the field of immune cell culture, and in particular relates to a method for culturing immune cells. Background technique [0002] At present, cancer treatment is mainly based on surgery, radiotherapy and chemotherapy, and the possibility of recurrence is high. In order to better protect the patient's body function, the doctor will try to preserve the organ function as much as possible during the operation, but the residual tumor cells may become a hidden danger of recurrence. Radiotherapy and chemotherapy can also have a great damaging effect on normal human cells. The technology amplifies and purifies the patient's immune cells in advance, and when the patient has undergone surgery, radiotherapy and chemotherapy, supplementing the immune cells can greatly improve the survival rate and quality of life of the patient. [0003] Immune cells are now widely used in clinics. Traditional immune cell culture takes about 10 days to ca...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12N5/0783
CPCC12N5/0634C12N5/0638
Inventor 朱兵锐刘朋程
Owner SHANGHAI HUAYAN MEDICINE TECH CO LTD
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