Set of immune culture triple bags and application thereof

A triple bag and culture medium technology, which is applied in tissue cell/virus culture devices, blood/immune system cells, bioreactor/fermenter combination, etc., can solve the problem of high detection cost, bacterial infection risk, and culture bag risk control lower problem

Inactive Publication Date: 2019-10-08
SHANGHAI HUAYAN MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Now that immune cells are widely used in clinical practice, the risk control of traditional culture bags is relatively low, the cost of later detection is high, and there is still a risk of bacterial infection

Method used

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  • Set of immune culture triple bags and application thereof
  • Set of immune culture triple bags and application thereof
  • Set of immune culture triple bags and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Utilize the immunoculture triple bag of the present invention to cultivate cik cells

[0032] Reagent: serum-free basal medium, purchased from Nataka Company, specification 500mL / bottle;

[0033] IFN-r, purchased from Cloning Gamma, with a specification of 1 million IU / bottle, and a concentration of 800-1000 IU / mL;

[0034] IL-2, purchased from Delusen, with a specification of 1 million IU, and a concentration of 400-500 IU / mL;

[0035] CD3mAb was purchased from Baori Medical, the specification was 1 mg / mL, and the concentration used was 10-20 ng / mL.

[0036] Methods and steps:

[0037] 1. Sample processing - GMP thousand-level laboratory operation:

[0038] 1.1 Fresh peripheral blood was collected as a sample blood sample, and the sample blood sample was first centrifuged in the first step (2500rpm / min, 15min) to separate plasma and cells.

[0039] 1.2 Extinguish the plasma centrifuged in step 1.1 (56°C, 30min).

[0040] 1.3 The cells in step 1.1 were separated by f...

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Abstract

The invention provides a set of immune culture triple bags and application thereof, and relates to the technical field of biological consumables. The immune culture triplet bags comprise a cell culture bag (1), a first culture medium storage bag (2), a second culture medium storage bag (3), a communication tube (4), a three-way stop valve (5),and a plurality of stop clamps (6); a sampling opening(7) and a connecting opening (8) are formed in the cell culture bag (1); a liquid inlet opening (9) and a communication opening (10) are formed in each of the first culture medium storage bag (2) andthe second culture medium storage bag (3); and the three-way stop valve (5) is detachably connected to the communication tube (4). The immune culture triplet bags are used in comparison with a culturebottle method, the 13d culture expansioncapacity is improved by 33.6%, the motility rate is increased by 21% and can reach 98%, and thus the immune culture triplet bags can be used for cell expansionability improvement and sterile culture.

Description

technical field [0001] The invention belongs to the technical field of biological consumables, and in particular relates to a set of immunoculture triple bags and applications thereof. Background technique [0002] At present, cancer treatment is mainly based on surgery, radiotherapy and chemotherapy, and the possibility of recurrence is high. In order to better protect the patient's body function, the doctor will try to preserve the organ function as much as possible during the operation, but the residual tumor cells may become a hidden danger of recurrence. Radiotherapy and chemotherapy can also have a great damaging effect on normal human cells. The technology amplifies and purifies the patient's immune cells in advance, and when the patient has undergone surgery, radiotherapy and chemotherapy, supplementing the immune cells can greatly improve the survival rate and quality of life of the patient. [0003] Now that immune cells are widely used in clinical practice, the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12N5/0783
CPCC12M23/14C12M23/20C12M37/04C12M99/00C12N5/0638
Inventor 朱兵锐刘朋程
Owner SHANGHAI HUAYAN MEDICINE TECH CO LTD
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