Regeneration method of 'Da-ding-xiang' litchi variety in vitro

Abstract

The invention discloses a regeneration method of 'Da-ding-xiang' litchi variety in vitro. The regeneration method of the 'Da-ding-xiang' litchi variety in vitro comprises the following steps: 1) carrying out callus cultivation by taking litchi anther as an explant so as to obtain embryogenic calluses; 2) performing somatic embryo induction culture on the embryogenic calluses by using a medium, which consists of IAA, ABA, 6-BA, inositol, lactoalbumin hydrolysate and coconut water, so as to obtain somatic embryos; 3) performing maturation culture on the somatic embryos by using a medium, which contains coconut water, so as to obtain mature somatic embryos; and 4) performing regeneration culture on the mature somatic embryos by using a medium, which contains 6-BA and GA3, so as to obtain regenerated plants. According to the invention, a culture method of the regenerated plants of the 'Da-ding-xiang' litchi variety in vitro is established so as to achieve very high somatic embryo inductionrate and germination rate, so that the culture method is capable of improving production efficiency and reducing cost; and thus, a good foundation is laid for litchi variety improving and breeding bybiotechnology.

Description

technical field [0001] The present invention relates to a method for in vitro regeneration of 'Dadingxiang' lychee variety, specifically, the present invention relates to a culture method for 'Dadingxiang' lychee variety to develop into a complete in vitro regenerated plant from callus induction through somatic embryogenesis. Background technique [0002] Litchi (Litchi chinensis Sonn.) is one of the important economic crops in my country and plays an important role in agricultural production in tropical regions. Problems such as unstable flowering and fruit setting of litchi, serious damage from diseases and insect pests, and difficulty in post-harvest preservation seriously restrict the development of litchi industry, and cultivating fine varieties of litchi is the fundamental way to solve these problems. [0003] The high genetic heterozygosity of litchi and the sterility of some varieties limit the application of sexual hybridization in litchi variety improvement. Trans...

AI Technical Summary

Problems solved by technology

At present, there is genotype dependence in in vitro regeneration of litchi, low frequency of somatic embryos, many deformed embryos and asynchronous height of somatic embryos, and low plant regeneration frequency, which is the technical bottleneck for establishing efficient in vitro regeneration of litchi

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