A method for in vitro regeneration of "Feizixiao" litchi variety

Abstract

The invention discloses a method for in vitro regeneration of a Feizixiao litchi variety. The method comprises the following steps: step 1, taking the anther of the Feizixiao litchi variety as an explant for callus induction and culture, and screening to obtain embryogenic callus; the culture medium is added with 2, 4-D with final concentration of 3 mg / l, BA with final concentration of 0.5 mg / l, NAA with final concentration of 0.5 mg / l and sugar with final concentration of 30 g / L on the basis of a culture medium MS; step 2, carrying out somatic embryo induction and culture to the embryogenic callus to obtain a somatic embryo; the culture medium is added with NAA with final concentration of 0.1 mg / l, LKT with final concentration of 5 mg / l, inositol with final concentration of 100 mg / l and sucrose with final concentration of 50 g / L on the basis of the culture medium MS; step 3, carrying out maturation to the somatic embryo to obtain a mature embryo, culture mediums are MS, 50 ml / L coconut milk and 60 g / L sucrose; and step 4, carrying out regeneration culture to the mature embryo, the culture medium is added with sucrose with final concentration of 30 g / L and agar with final concentration of 7 g / L on the culture medium MS. The invention establishes a method for in vitro plant regeneration of a Feizixiao litchi variety, achieves the high induction rate, improves the production efficiency, reduces the cost and lays a good foundation for litchi variety improvement and biotechnology breeding.

Description

Technical field [0001] The present invention relates to a method for in vitro regeneration of the'Feizixiao' litchi variety. Specifically, the present invention relates to a culture method for the'Feizixiao' litchi variety to induce in vitro embryogenesis from callus and develop into a complete in vitro regeneration plant. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a major tropical and subtropical fruit tree in southern my country. It occupies an important position in tropical agricultural production and is an important part of farmers' income sources. With the improvement of people's living standards, the pursuit of commodity value is also getting higher and higher, and the demand for accelerating litchi breeding technology and new varieties cultivation is imminent. Litchi has a long juvenile period and highly heterozygous genetic composition. Traditional breeding techniques to improve litchi traits and cultivate new varieties are time-consuming, laborious,...

AI Technical Summary

Problems solved by technology

[0003] Since Fu Lianfang et al. reported that litchi tissue culture obtained complete plants in 1983, researchers have carried out tissue culture research one after another, but the progress is relatively slow

At present, the bottleneck of litchi biotechnology breeding lies in the failure to establish an efficient genetic transformation system, and the establishment of an in vitro regeneration system for litchi is the premise and basis for the establishment of an efficient genetic transformation system

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