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Medicinal composition and application thereof

A composition and drug technology, applied in the field of biomedicine, to achieve the effects of preventing and treating myocardial injury, expanding the scope, and protecting the mitochondria of myocardial cells

Inactive Publication Date: 2020-04-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether caffeic acid has a protective effect on myocardial injury caused by doxorubicin and its specific mechanism have not been reported in the literature.

Method used

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  • Medicinal composition and application thereof
  • Medicinal composition and application thereof
  • Medicinal composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Effect of caffeic acid on viability of cardiomyocytes injured by doxorubicin

[0024] The one-day-old SD suckling mice were taken out, killed by disinfection, and the apex of the heart was taken, and the cardiomyocytes were obtained by routine digestion with trypsin. Suspend cardiomyocytes in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 After culturing for 2 h, the obtained cardiomyocytes were purified. according to 10 5 cells / mL were inoculated in a 96-well plate and cultured for 48 h. The purified cardiomyocytes were randomly divided into 8 groups, which were normal control group, doxorubicin group (DOX), caffeic acid group (CA) and 5 groups of different concentrations of doxorubicin+caffeic acid mixed groups (DOX+CA). ). Doxorubicin was dissolved in normal saline, with a final concentration of 10 μM; caffeic acid was dissolved in preheated double-distilled water, with a final concentration of 30 μM, and then diluted to the required co...

Embodiment 2

[0038] Example 2 Effect of Caffeic Acid on Cardiomyocyte Injury Caused by Adriamycin

[0039] The cardiomyocytes grouped in Example 1 were 37 ° C, 5% CO 2 After incubation in the incubator for 24 hours, the cell supernatants of each group were extracted, and the content of lactate dehydrogenase (LDH) in each group was detected with an automatic biochemical analyzer. figure 1 is the comparison chart of the experimental results of myocardial cell injury in each group; figure 1 As shown, the LDH content in the supernatant of the DOX group was 36.87±1.86U / mL, much higher than that of the Control group (6.92±0.37U / mL), while the LDH content in each CA+DOX group was lower than that of the DOX group, and There was a certain dose dependence; the LDH content of the CA group and the Control group were similar, and compared with the Control group, * P # P <0.05. It can be seen that doxorubicin can cause myocardial cell damage or death, but caffeic acid has no effect on myocardial ce...

Embodiment 3

[0040] Example 3 Effect of Caffeic Acid on Cardiomyocyte Apoptosis Caused by Adriamycin

[0041] The cardiomyocytes grouped in Example 1 were 37 ° C, 5% CO 2After incubation in the incubator for 24 hours, the OD value reflecting the nucleosome content (nucleosome can reflect cell apoptosis) in the lysate was detected by ELISA method. Note: High-speed centrifugation is required after cell lysis, and the supernatant contains nucleosomes.

[0042] figure 2 It is a comparison chart of nucleosome content OD value in each group of cardiomyocyte lysates; figure 2 As shown, the OD value of the supernatant in the DOX group was 0.88±0.013, which was much higher than that in the Control group (0.38±0.013), while the OD values ​​in each CA+DOX group were lower than those in the DOX group, showing a certain dose-dependence ; CA group and Control group OD values ​​are close, compared with Control group, * P # P <0.05. It can be seen that doxorubicin leads to the apoptosis of cardio...

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PUM

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Abstract

The present invention relates to the field of biomedicines and particularly to a medicinal composition and an application thereof. The medicinal composition significantly reduces myocardial cell damage caused by doxorubicin and improves cell viability in SD neonatal rat myocardial cell injury tests; and after detecting mitochondrial ATPase activity of cardiomyocytes after combined administration,the combined application of caffeic acid with doxorubicin can enhance activity of Na<+>-K<+>-ATPase and Ca<2+>-ATPase in the cardiomyocytes. The combined administration of the caffeic acid with doxorubicin can prevent and treat myocardial damage caused by the doxorubicin, enhances viability of myocardial cells after injury, reduces degree of myocardial cell injury and apoptosis rates, protects mitochondria of the myocardial cells, reduces side effects of the doxorubicin, and expands scopes of clinical applications of the doxorubicin.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a pharmaceutical composition and its application. Background technique [0002] Doxorubicin (DOX), also known as doxorubicin, is an anthracycline tumor antibiotic that can inhibit the synthesis of DNA and RNA, has a wide anti-tumor spectrum, and can kill tumor cells of various growth cycles effect. It is mainly applicable to the treatment of solid tumors such as acute leukemia, malignant lymphoma and breast cancer. However, in the course of clinical application, it can cause severe damage to cardiomyocytes, which will seriously affect the health of patients, and even threaten the lives of patients. Therefore, how to develop new drugs that inhibit the toxicity of doxorubicin in cardiomyocytes has always been an important issue in medicine. [0003] Caffeic acid (CA) is a natural phenolic compound that widely exists in plants and can be used safely in cosmetics. It has a wide range of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61K31/704A61P35/00A61P9/00
CPCA61K31/192A61K31/704A61P35/00A61P9/00A61K2300/00
Inventor 高恶斌刘坤叶鹏林朱杨杰
Owner JIANGSU UNIV
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