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Method for expanding culture of brachionus calyciflorus in half environmental capacity

A calyx flower arm tail, environmental capacity technology, applied in fish farming, application, animal husbandry and other directions, can solve problems such as unfavorable researchers' experimental research, and achieve the effects of ensuring stable acquisition, significant effects, and simple methods

Inactive Publication Date: 2020-04-28
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such wild fluctuations in population density are not conducive to researchers conducting experimental studies

Method used

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  • Method for expanding culture of brachionus calyciflorus in half environmental capacity
  • Method for expanding culture of brachionus calyciflorus in half environmental capacity
  • Method for expanding culture of brachionus calyciflorus in half environmental capacity

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Embodiment 1

[0013] The purpose of the present invention is to provide a method for expanding the half of the environmental capacity of Brachionus calyxae.

[0014] The method adopts EPA culture medium to cultivate Brachionus calyciflora. The day and night cycle of light is 12h:12h, and the light intensity is 1500Lx. The food is Chlorella (Institute of Hydrobiology, Chinese Academy of Sciences #1067) cultivated by BG-11, and the addition amount is 10 6 cell / ml.

[0015] The method of the invention carries out transfer and expansion cultivation when the rotifer fecundity is the most vigorous, which is beneficial to maintaining the continuous cultivation of the rotifer population, and ensures the stability and reliability of the rotifer as a source of experimental materials.

[0016] In order to achieve the above object, the method comprises the following steps:

[0017] 1. Determining the K point of the growth rate of Brachionus calyciflora

[0018] (1) Incubation of resting eggs of Bra...

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Abstract

The invention belongs to the technical field of relationships between fishery biological community structures and food web functions. The invention provides a method for expanding culture of brachionus calyciflorus in half environmental capacity. The method comprises determining growth rate K point for culture of brachionus calyciflorus and culturing brachionus calyciflorus at K / 2 point. The method of the present invention offers transfer culture at K / 2 point to maintain long-term high-speed stable growth of the population, facilitates to ensure stable acquisition of experimental materials, namely the transfer culture is conducted in the most vigorous stage of growth and reproduction, thereby keeping stable growth of the brachionus calyciflorus population. The method of the invention is simple and easy to use, easily available in materials, remarkable in effect and suitable for stable cultivation of the brachionus calyciflorus.

Description

technical field [0001] The invention belongs to the technical field of fishery biological community structure and food web function relationship. Background technique [0002] Rotifers belong to the phylum Rotifera, and most species have a body size of 100-500 μm, which is a very prosperous group among pseudocoelomates. Its number of species is second only to nematodes, and there are about 2,000 species in the world. Most of the rotifers (about 1,600 species) belong to the class Mononidel, and the rest belong to Dinidel (another classification method is divided into Hindromorpha and Caudoidea). Many species of rotifers are widely distributed throughout the world, most of which live in freshwater environments. [0003] Brachionus calyciflorus plays an important role in freshwater rotifers. Brachionus calyx belongs to class Monogononta, order Ploima, family Brachionidae, and genus Brachionus. Brachionus calyxalis has the advantages of fast growth, wide distribution in my c...

Claims

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Application Information

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IPC IPC(8): A01K61/40
CPCA01K61/40
Inventor 张欢何雨晗姚慧徐军
Owner INST OF AQUATIC LIFE ACAD SINICA
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