PCR primer group for detecting pepper mild mottle virus, test kit and application thereof

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) primer group for detecting pepper mild mottle virus, the PCR primer group comprises three pepper mild mottle virus (PMMoV) genome motor protein (MP) and coat protein (CP) coding gene partial sequence specific primers and two pepper reference gene Actin specific primers, wherein the three pepper mild mottle virus (PMMoV) genome motor protein(MP) and coat protein (CP) coding gene partial sequence specific primers are PMMoV-F1, PMMoV-F470 and PMMoV-R991 respectively, and the partial sequence specific primers of the MP gene and the CP geneare PMMoV-F470 and PMMoV-R991; the two pepper reference gene Actin specific primers are PeActin-F1 and PeActin-R1093. The PCR amplification result is detected in 1.0%-1.5% agarose, an expensive fluorescent quantitative PCR instrument is not needed, and the PCR primer group has the advantages of being rapid, economical, high in stability, high in accuracy and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of crop virus detection, and in particular relates to a PCR primer set, a kit and an application thereof for detecting pepper mild mottle virus (PMMoV). Background technique [0002] Pepper mild mottle virus (PMMoV) is a (+) sRNA virus of the genus Tobamovirus, which was first discovered in the United States and later reported in Germany, the Netherlands, the United Kingdom, Canada, Australia and other countries . The virus is mainly transmitted through seeds and sap, and the vector is not easy to transmit the virus. The infected seeds, infected plants and infected soil are important sources of infection, and can survive for 25 years on dry plant residues. PMMoV infection of pepper plants can cause leaf shrinkage, mottled and mosaic symptoms, fruit chlorosis and mottled, smaller, deformed and even local necrosis and shedding, causing serious harm to pepper production. In 1994, the harm of the virus was fir...

AI Technical Summary

Problems solved by technology

However, the current RT-PCR methods only use a pair of specific primers to detect PMMoV, so that false negatives cannot be ruled out, and false positives may even occur, resulting in inaccurate detection results.

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