Microbial fertilizer for improving saline-alkali soil and preparation method and application thereof
A technology of microbial fertilizer and saline-alkali soil, which is applied in botany equipment and methods, preparation and application of organic fertilizers, etc. It can solve the problem of soil pH, high salt content, improvement of properties of saline-alkali soil, improvement of physical and chemical methods, etc. problems, achieve the effect of improving soil structure, reducing soil compaction, and inhibiting the effect of returning salt
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[0053] like figure 1 As shown, the preparation method of the improved saline-alkali land microbial fertilizer provided by the embodiments of the present invention specifically includes:
[0054] S101, preparing microbial fermentation liquid, corn stalks, urea, and calcium phosphate according to the weight percentage; crushing the corn stalks with a micro-powder prototype machine for later use.
[0055] S102, activate wp-3 strain in LB solid medium.
[0056] S103, inoculate the activated strain into the fermentation broth adjusted to pH 7.5-7.83L with ammonia water and ferment for 72 hours, the total number of live bacteria of the strain shall not be less than 2×10 8 CFU / mL.
[0057] S104, adding 150g of urea and 30g of calcium phosphate to the fermentation broth obtained in step S103.
[0058] S105, pour the solution obtained in step S104 into 200 g of corn stalk powder, place it in a fermentation bag, and after fermentation for 5-7 days, air-dry it naturally until the wate...
Embodiment 1
[0062] The invention provides an improved microbial fertilizer for saline-alkali land, which consists of the following components by weight percentage: 85-90% of microbial fermentation liquid, 6-7% of corn straw, 4-5% of urea and 1-2% of calcium phosphate.
[0063] The preparation method of above-mentioned microbial fertilizer, comprises the following steps:
[0064] The activated strain wp-3 was inoculated in the fermentation broth, and fermented at 30° C. in a shaker at 180 r / min for 72 hours.
[0065] Fermentation broth: 5g of yeast extract, 10g of tryptone, 1000mL of distilled water, adjusted to pH 7.5-7.8 with ammonia water, and sterilized at 121°C for 20 minutes.
[0066] Add 150g urea and 30g calcium phosphate to the above-mentioned fermented liquid.
[0067] 200 g of corn stalks were pulverized with a micro-powder sample machine and placed in a fermentation bag.
[0068] Pour the above fermented liquid with urea and calcium phosphate into a fermenting bag, finish fer...
Embodiment 2
[0070] The application of the saline-alkali soil microbial fertilizer provided by the embodiment of the present invention in wheat growth and saline-alkali soil improvement. include:
[0071] (1) Wheat pot experiment
[0072] The soil and the microbial fertilizer were mixed at a ratio of 3:1 to treat the bacterial fertilizer, and the soil without bacterial fertilizer was set as the control.
[0073] Add 2% sodium chloride to the soil without bacterial manure and the soil with bacterial manure.
[0074] Wheat seeds were sown in pots containing an equal amount of 2kg of soil, 20 seeds per pot, and 3 replicates were set for the control and treatment. Timely and equal watering, thinning after 7 days, keeping 6 plants with uniform growth in each pot, measuring their growth and soil physical and chemical properties after 30 days. The effect of promoting growth is as follows figure 2 .
[0075] (2) Determination of wheat growth indicators
[0076] Microbial fertilizer can prom...
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