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Protein chip for combined detection of multiple thrombus markers

A protein chip and marker technology, applied in the biological field, can solve the problems of cumbersome operation, high cost, and low efficiency, and achieve the effects of improving detection efficiency, reducing detection cost, and low cost

Pending Publication Date: 2020-04-17
江苏三联生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0027] Aiming at the problems of high cost, low efficiency and cumbersome operation in the prior art, the applicant of the present invention provides a protein chip for detection of new thrombus markers and its preparation method

Method used

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  • Protein chip for combined detection of multiple thrombus markers
  • Protein chip for combined detection of multiple thrombus markers
  • Protein chip for combined detection of multiple thrombus markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A protein chip for detection of various thrombus markers, the preparation method of the protein chip comprises the following steps:

[0052] (1) Black slide pretreatment;

[0053] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 16 hours, and then wash it with purified water for 2 to 8 times;

[0054] ② Soak the black slide in 0.05% silane aqueous solution for 60 minutes;

[0055] ③Put the soaked black glass slide into an oven and bake at 180°C for 0.2h.

[0056] (2) Antibody solution spotting;

[0057] refer to figure 1 , Using machine automation to spot TAT monoclonal antibody solution, PIC monoclonal antibody solution, tPAIC monoclonal antibody solution, TM monoclonal antibody solution, FDP monoclonal antibody solution, DD monoclonal antibody solution. The concentration of the antibody solution is 0.1mg / mL, and 20nL is applied to each point, and the distribution of each antibody is as follows: figure 1 shown.

[0058] (3) closed ...

Embodiment 2

[0063] A protein chip for detection of various thrombus markers, the preparation method of the protein chip comprises the following steps:

[0064] (1) Black slide pretreatment;

[0065] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 24 hours, and then wash it with pure water for 2 to 8 times;

[0066] ② Soak the black slide in 0.5% silane aqueous solution for 30 minutes;

[0067] ③Put the soaked black glass slide into an oven and bake at 140°C for 0.5h.

[0068] (2) Antibody solution spotting;

[0069] refer to figure 1 , Using machine automation to spot TAT monoclonal antibody solution, PIC monoclonal antibody solution, tPAIC monoclonal antibody solution, TM monoclonal antibody solution, FDP monoclonal antibody solution, DD monoclonal antibody solution. The concentration of the antibody solution is 0.1mg / mL, and 20nL is applied to each point, and the distribution of each antibody is as follows: figure 1 shown.

[0070] (3) closed proce...

Embodiment 3

[0075] A protein chip for detection of new thrombus markers, the preparation method of the protein chip comprises the following steps:

[0076] (1) Black slide pretreatment;

[0077] ① Soak the black slide in the slide pretreatment solution containing 2% NaOH for 20 hours, and then wash it with pure water for 2 to 8 times;

[0078] ② Soak the black slide in 1% silane aqueous solution for 20 minutes;

[0079] ③Put the soaked black glass slide into an oven and bake at 100°C for 0.6h.

[0080] (2) Antibody solution spotting;

[0081] refer to figure 1 , Using machine automation to spot TAT monoclonal antibody solution, PIC monoclonal antibody solution, tPAIC monoclonal antibody solution, TM monoclonal antibody solution, FDP monoclonal antibody solution, DD monoclonal antibody solution. The concentration of the antibody solution is 0.1mg / mL, and 20nL is applied to each point, and the distribution of each antibody is as follows: figure 1 shown.

[0082] (3) closed process;

...

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Abstract

The invention discloses a protein chip for combined detection of multiple thrombus markers. A preparation method of the protein chip comprises the following steps: (1) black slide pretreatment; (2) antibody solution sample application; (3) a sealing process; and preparation of the protein chip. The protein chip is used for detecting concentrations of various thrombus markers, preventing and reducing thrombus risks and guiding thrombus treatment. The product disclosed by the invention is a protein chip diagnostic kit comprising six indexes of TAT (thrombin-antithrombin complex), PIC (plasmin-alpha2 anti-plasminogen complex), tPAIC (tissue-type plasminogen activator-plasminogen activation inhibition complex), TM (thrombomodulin), FDP (fibrinogen degradation product) and DD (D-dimer). The indexes are detected by using a protein chip technology for the first time, and the method has the advantages of quickness, high efficiency, simplicity, convenience, low cost and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein chip for detecting multiple thrombus markers and a preparation method thereof. Background technique [0002] The seven blood coagulation items and coagulation factor screening are basically passive detection after thrombosis, but are not sensitive to pre-thrombus formation. Compared with thrombolytic therapy after thrombus is discovered, it is particularly important to detect the risk of thrombus in time before thrombus formation and take timely measures. Aiming at the problem of clinical diagnosis before and in the early stage of thrombosis, Sysmex Corporation of Japan proposed for the first time four new thrombosis items (TAT, TM, PIC, tPAIC), plus the combination of the two indicators of FDP and DD of the fibrinolytic system Detection can play a role in early detection and early treatment of thrombus. [0003] At present, the detection methods of the four markers of ne...

Claims

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Application Information

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IPC IPC(8): G01N33/552G01N33/531G01N33/68
CPCG01N33/531G01N33/552G01N33/6893G01N2800/226
Inventor 朱旭国泮锋纲丁俊杰施启尧
Owner 江苏三联生物工程股份有限公司
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