Method for ultrasonic-assisted sugaring-out extraction of plant seeds rich in grease and flavone

A plant seed and ultrasonic technology, applied in the field of bioengineering, can solve the problems of reducing the comprehensive utilization rate of plant seeds, single extraction, and increasing costs, and achieve the effects of improving comprehensive utilization rate, short phase separation time, and low equipment investment cost

Pending Publication Date: 2020-04-17
HEXI UNIV
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Problems solved by technology

[0002] In the prior art, the extraction of flavonoids in plant seeds generally adopts ultrasonic-assisted extraction, traditional solvent extraction and salting-out extraction; but in the prior art, the extraction of plant seeds rich in oil and flavonoids is a single extraction, which not only waste resources, increase the cost, and reduce the comprehensive utilization rate of plant seeds; therefore, finding an efficient, economical method for simultaneously extracting plant seeds rich in oil and flavonoids has become the focus of technical research

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  • Method for ultrasonic-assisted sugaring-out extraction of plant seeds rich in grease and flavone

Examples

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Effect test

Embodiment 1

[0019] This embodiment provides a method for ultrasonic-assisted sugar separation and extraction of oil and flavonoids in borage, the method comprising the following steps:

[0020] A. Ultrasonic-assisted sugar separation extraction: Weigh 4 grams of crushed borage, add 34 grams of tert-butanol, 22 grams of maltose and 30 grams of double-distilled water and mix evenly to obtain a mixed solution. The mixed solution is heated at an ultrasonic power of 100W , the ultrasonic frequency is 25KHz, the ultrasonic work is 1s, the gap is 2s, the extraction temperature is 30°C, the extraction time is 10min, let stand, and the mixture is divided into upper phase and lower phase;

[0021] B. Recovery of grease: the upper phase prepared in step A is concentrated under reduced pressure to obtain grease;

[0022] C. Recovery of flavonoids: add 120 grams of absolute ethanol to the lower phase prepared in step A, let it stand at 3°C ​​for 3 hours, and centrifuge at a speed of 5000r / min for 8min...

Embodiment 2

[0024] The embodiment provides a method for ultrasonic-assisted sugar separation and extraction of oil and flavonoids in black beans, the method comprising the following steps:

[0025] A. Ultrasonic-assisted sugar extraction: Weigh 8 grams of crushed black soybeans, add 40 grams of tert-butanol, 30 grams of maltose and 30 grams of double-distilled water and mix evenly to obtain a mixed solution. The mixed solution is heated at an ultrasonic power of 450W. The ultrasonic frequency is 40KHz, the ultrasonic work is 3s, the interval is 5s, the extraction temperature is 25°C, the extraction time is 15min, let stand, and the mixture is divided into upper phase and lower phase;

[0026] B. Recovery of grease: the upper phase prepared in step A is concentrated under reduced pressure to obtain grease;

[0027] C. Recovery of flavonoids: add 135 grams of absolute ethanol to the lower phase prepared in step A, let it stand at 5°C for 2 hours, and centrifuge at 6000r / min for 10min to obt...

Embodiment 3

[0029] This embodiment provides a method for ultrasonically assisted sugar separation and extraction of oil and flavonoids in sunflower seeds, the method comprising the following steps:

[0030] A. Ultrasonic-assisted sugar extraction: Weigh 2 grams of crushed sunflower seeds, add 20 grams of tert-butanol, 15 grams of maltose and 65 grams of double-distilled water and mix evenly to obtain a mixed solution. The mixed solution is heated at an ultrasonic power of 600W , the ultrasonic frequency is 50KHz, the ultrasonic work is 4s, the gap is 8s, the extraction temperature is 40°C, the extraction time is 3min, let stand, and the mixture is divided into upper phase and lower phase;

[0031] B. Recovery of grease: the upper phase prepared in step A is concentrated under reduced pressure to obtain grease;

[0032] C. Recovery of flavonoids: Add 145 grams of absolute ethanol to the lower phase prepared in step A, let it stand at 4°C for 1 hour, and centrifuge at 8000r / min for 5min to ...

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Abstract

The invention provides a method for ultrasonic-assisted sugaring-out extraction of plant seeds rich in grease and flavone. The method comprises the following steps: A, ultrasonic-assisted sugaring-outextraction: crushing plant seeds, uniformly mixing the crushed plant seeds with an organic solvent, sugar and double distilled water to prepare a mixed solution, carrying out ultrasonic treatment onthe mixed solution, and conducting standing to allow the mixed solution to be layered into an upper phase and a lower phase; B, grease recovery: concentrating the upper phase prepared in the step A under reduced pressure to prepare the grease; C, flavone recovery: mixing the lower phase prepared in the step A with absolute ethyl alcohol, carrying out standing at low temperature, performing centrifuging to obtain a supernatant and a precipitate, and distilling the supernatant under reduced pressure to obtain the flavone. The ultrasonic-assisted sugaring-out extraction technology provided by theinvention has the advantages that extraction efficiency is improved, yield is high, equipment investment cost is relatively low, a comprehensive utilization rate is improved, cost is reduced, and both the organic solvent and the sugar can be recycled.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and particularly relates to a method for extracting plant seeds rich in oil and flavonoids through ultrasonic-assisted sugar separation. Background technique [0002] In the prior art, the extraction of flavonoids in plant seeds generally adopts ultrasonic-assisted extraction, traditional solvent extraction and salting-out extraction; but in the prior art, the extraction of plant seeds rich in oil and flavonoids is a single extraction, which not only wastes resources, increase the cost, and reduce the comprehensive utilization of plant seeds; therefore, finding an efficient, economical method for simultaneously extracting plant seeds rich in oil and flavonoids has become the focus of technical research. Contents of the invention [0003] In order to solve the above technical problems, the present invention provides a method for extracting plant seeds rich in oil and flavonoids through ul...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10B01D11/02
CPCC11B1/04C11B1/106C11B1/108B01D11/0265
Inventor 张喜峰罗光宏张挺峰王丽娟王鑫鑫张青婷
Owner HEXI UNIV
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