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Application of Senecio scandens extract in preparation of medicine for relaxing airway smooth muscles

A technology of airway smooth muscle and telepathy, which is applied in the field of medicine to achieve the effect of efficiently relaxing airway smooth muscle and relieving the symptoms of airway obstruction

Inactive Publication Date: 2020-04-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad about the research on the relaxation of tracheal smooth muscle and the treatment of asthma by Senecio extract.

Method used

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  • Application of Senecio scandens extract in preparation of medicine for relaxing airway smooth muscles
  • Application of Senecio scandens extract in preparation of medicine for relaxing airway smooth muscles
  • Application of Senecio scandens extract in preparation of medicine for relaxing airway smooth muscles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Senecio Extract

[0019] Take the dried tubers of Senecio senecio, crush them, add 65-75% ethanol at a ratio of 1:9 to 11, soak at room temperature for 1 week, reflux and extract to obtain the extract, and evaporate the extract under reduced pressure to obtain the crude extract of Senecio ( (I.e. Senecio ethanol extract), extracting and layering the Senecio ethanol extract with petroleum ether to obtain a petroleum ether layer and a first water layer, and concentrating the petroleum ether layer under reduced pressure to obtain the Senecio petroleum ether extract; The first aqueous layer is extracted with chloroform and separated to obtain a chloroform layer and a second aqueous layer. The chloroform layer is concentrated under reduced pressure to obtain a senecio chloroform extract; the second aqueous layer is extracted with ethyl acetate to obtain acetic acid. The ethyl acetate layer and the third aqueous phase. The ethyl acetate layer is concentr...

Embodiment 2

[0021] Example 2 Senecio extract relaxes the pre-contraction of airway smooth muscle induced by high potassium

[0022] 1. In vitro isolation of mouse trachea:

[0023] Male adult BALB / c mice of similar age were sacrificed by cervical dislocation. The mice were fixed on the dissecting board with the abdomen facing upwards, and the thoracic cavity was cut to expose the heart and lung tissues. After removing the heart, remove the one from the laryngeal knot to the lungs. The entire tissue, immediately soak the removed tissue in PSS solution (NaCl 135mM, KCl 5mM, MgCl 2 1mM, CaCl 2 2mM, HEPES 10mM, glucose 10mM, NaOH adjusted to pH 7.4), and then transferred to the gel plate to further separate the tracheal ring. Under a stereo microscope, use Venus scissors to carefully remove the connective tissue around the trachea and the residual blood in the lumen, and separate the main tracheal ring about 4-5mm.

[0024] 2. Method of detecting the tension of airway smooth muscle in mice:

[002...

Embodiment 3

[0031] Example 3 Senecio extract relaxes acetylcholine-induced airway smooth muscle pre-contraction

[0032] After the balance is completed, the 80mM high potassium solution (H-K + ) Is replaced with 100 μM acetylcholine (ACh) solution for pre-stimulation, and the rest of the steps are the same as in Example 2.

[0033] The result is figure 2 As shown, it indicates that the isolated mouse tracheal ring undergoes stable and continuous contraction in 100μM ACh solution. After the concentration-dependent addition of Senecio extract, the contracted tracheal ring tension gradually decreases until it drops to the baseline level before contraction; control group The tracheal ring that does not cause pre-contraction to relax. It shows that Senecio extract can substantially completely relax acetylcholine and cause airway smooth muscle contraction, and the drug's effect is concentration-dependent. From Table 2 and figure 2 It can be seen that the relaxation rate of senecio extract at a co...

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Abstract

The invention discloses application of a Senecio scandens extract in the preparation of a medicine for relaxing airway smooth muscles. Research finds that the Senecio scandens extract is found to be amedicine with an efficient airway smooth muscle relaxing effect by detecting the tension of the isolated tracheal ring muscle of an experimental mouse, which indicates that the Senecio scandens is asource for developing an efficient new medicine for preventing and treating asthma, and can be applied to relieving the symptom of airway obstruction when an asthma patient is attacked. The research of the invention finds that the Senecio scandens extract inhibits calcium ion internal flow by blocking at least one of an LVDCC ion channel or an NSCCs ion channel so as to relax preshrunk airway smooth muscles.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to the application of senecio extract in the preparation of a medicine for relaxing airway smooth muscle. Background technique [0002] Asthma is a chronic inflammatory disease of the airway, which is mainly involved in a variety of inflammatory cells such as eosinophils, mast cells and T lymphocytes. The pathogenesis of asthma is very complicated. Although its etiology and pathogenesis have not yet been fully understood, one of its main pathophysiological characteristics is airflow obstruction, airway hyperresponsiveness, and airflow limitation with periodic attacks. In recent years, the morbidity and mortality of asthma have been increasing. About 300 million people worldwide are deeply affected by asthma. The World Health Organization estimates that asthma has caused about 383,000 deaths in the world, and it has brought a huge impact to patients and society. Health and ec...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P11/06
CPCA61K36/28A61P11/06
Inventor 彭勇波杨卉卉刘庆华沈金花
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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