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Application of Baiwei extract in preparation of medicine for relaxing airway smooth muscle

An airway smooth muscle relaxation technology, applied in the field of medicine, to achieve the effect of efficiently relaxing airway smooth muscle and relieving the symptoms of airway obstruction

Inactive Publication Date: 2022-02-11
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no reports at home and abroad about the study of Baiwei extract to relax tracheal smooth muscle in the treatment of asthma

Method used

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  • Application of Baiwei extract in preparation of medicine for relaxing airway smooth muscle
  • Application of Baiwei extract in preparation of medicine for relaxing airway smooth muscle
  • Application of Baiwei extract in preparation of medicine for relaxing airway smooth muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of embodiment 1 Baiwei extract

[0019] Take the dried tuber of Baiwei medicinal material, crush it, add 50% ethanol according to the ratio of 1:10, soak at room temperature for 1 week, reflux extraction to obtain the extract, and evaporate the extract to dryness under reduced pressure to obtain the crude extract of Baiwei (that is, Baiwei ethanol extract), the ethanol extract of Baiwei and petroleum ether are extracted and layered to obtain a petroleum ether layer and the first water layer, and the petroleum ether layer is concentrated under reduced pressure to obtain Zebaiwei petroleum ether extract; The first aqueous layer was extracted with chloroform and separated to obtain a chloroform layer and the second aqueous layer, and the chloroform layer was concentrated under reduced pressure to obtain a chloroform extract of Baiwei; the second aqueous layer was extracted with ethyl acetate to obtain ethyl acetate. layer and a third aqueous phase, the ethy...

Embodiment 2

[0020] Example 2 Baiwei extract relaxes the pre-contraction of airway smooth muscle induced by high potassium

[0021] 1. In vitro isolation of mouse trachea:

[0022] Male adult BALB / c mice of similar age were sacrificed by cervical dislocation, and the mice were fixed on the dissecting board with the abdomen facing up, and the chest cavity was cut open to expose the heart and lung tissue. After the heart was removed, a section from the Adam's apple to the lung For the whole tissue, immediately soak the removed tissue in PSS solution (NaCl 135mM, KCl 5mM, MgCl 2 1mM, CaCl 2 2mM, HEPES10mM, glucose10mM, NaOH to adjust the pH to 7.4), and then transferred to the gel plate to further separate the tracheal ring. Under a stereomicroscope, use Venus scissors to carefully remove the connective tissue around the trachea and the residual blood in the lumen, and separate the main tracheal ring of about 5-7mm.

[0023] 2. The tension detection method of mouse airway smooth muscle: ...

Embodiment 3

[0026] Example 3 Baiwei extract relaxes the pre-contraction of airway smooth muscle induced by acetylcholine

[0027] The remaining steps were the same as in Example 2, except that 80 mM high potassium solution (H-K) was replaced with 100 μM acetylcholine (ACh) solution for pre-stimulation after equilibration.

[0028] Such as figure 2 , the isolated mouse tracheal rings contracted stably and continuously in 100μMACh solution, and the tension of the contracted tracheal rings decreased gradually after adding the extract of Bai Wei in a concentration-dependent manner, until it dropped to the baseline level before contraction; the control group did not cause pre-contraction The tracheal ring dilates. It shows that the extract of Baiwei can basically completely relax the contraction of airway smooth muscle caused by acetylcholine, and the drug effect is concentration-dependent.

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Abstract

The invention discloses the application of Bai Wei extract in the preparation of medicines for relaxing airway smooth muscle; the present invention finds that Bai Wei extract has high-efficiency dilating airway muscle tension through the detection of muscle tension in isolated tracheal rings of experimental mice. Drugs that act on airway smooth muscle can be used to relieve the symptoms of airway obstruction in asthma patients, and have great promotion value.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of Baiwei extract in preparing medicine for relaxing airway smooth muscle. Background technique [0002] Asthma is a kind of chronic airway inflammation, which is participated by various inflammatory cells such as eosinophils, mast cells and T lymphocytes. The plausible physiologic features are periodic or episodic airflow obstruction, airway hyperresponsiveness, and airflow limitation. In the early stage of acute asthma attack, airway smooth muscle spasm is prominent, while airway inflammation is not significant. After 48 hours, it enters the chronic phase and begins to have typical airway inflammation characteristics. Therefore, mast cells are not important for delayed asthmatic responses. It is now believed that eosinophils are the key cells that cause airway inflammation, bronchial hyperresponsiveness and delayed asthmatic response, and are the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/27A61P11/06
CPCA61K36/27A61P11/06A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 柳梦苏刘庆华于孟飞
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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