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Bacillus megaterium and application thereof in degrading pyrethroid insecticides

A technology of Bacillus megaterium and pyrethroids, which is applied to Bacillus megaterium strain HLJ7 and the inoculants and application fields produced by it, can solve the problem of unstable microbial mineralization ability and degradation performance, polluted biodegradation, and no allethrin and other problems, to achieve the effect of enriching the germplasm resource pool and improving the degradation effect.

Active Publication Date: 2020-04-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the unstable mineralization ability and degradation performance of microorganisms to pesticides, the existing resource library of degrading bacteria is far from meeting the actual needs of biodegradation of chemical pesticide residue pollution.
In particular, there is no specific degradation preparation product for allethrin

Method used

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  • Bacillus megaterium and application thereof in degrading pyrethroid insecticides
  • Bacillus megaterium and application thereof in degrading pyrethroid insecticides
  • Bacillus megaterium and application thereof in degrading pyrethroid insecticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation, screening and identification of pesticide degradation bacterial strain HLJ7

[0033] 1. Enrichment culture and isolation and screening of allethrin-degrading bacteria:

[0034] The farmland soil in the suburbs of Harbin, Heilongjiang was collected, and 5 g of activated sludge samples were weighed and added to 50 mL of the above liquid basic medium containing allethrin (50 mg / L). After culturing at 30°C and 200rpm for 7 days, the mass concentration of pesticides was sequentially increased from 50mg / L to 100mg / L, 200mg / L, 400mg / L, and 800mg / L at an inoculum size of 10% each time for continuous enrichment culture. Then, the culture medium transferred for 4 times was serially diluted and spread on the LB solid plate containing 50 mg / L allethrin, and cultured upside down at 30°C for 2 days. After a single colony grows on the plate, the single colony is picked and streaked several times for purification, and a bacterium is isolated and numbered HLJ7.

...

Embodiment 2

[0040] Embodiment 2 Bacillus megaterium bacterial strain HLJ7 is to the degradability determination of allethrin

[0041] 1. Experimental method

[0042] (1) Seed liquid preparation:

[0043] The purified strain HLJ7 was inserted into 5 mL of LB liquid medium for overnight activation to the logarithmic phase. After centrifugation at 4° C., the bacteria were washed with physiological saline (0.9% NaCl), and the obtained bacteria were used as inoculum.

[0044] (2) Degradation performance determination:

[0045] Press 1.0×10 7 The inoculum amount of CFU / mL wet thalline was inoculated into 50 mL basal medium containing allethrin (50 mg / L) and inorganic salt medium respectively, and no inoculation was used as a control respectively, and each group was repeated three times. Incubate in a constant temperature shaker at 30°C and 200rpm for 11 days, take samples regularly, and measure OD with a spectrophotometer 600 The value represents the growth of the strain HLJ7, and its degra...

Embodiment 3

[0066] Example 3 Determination of the effect of Bacillus megaterium strain HLJ7 on degrading allethrin in soil

[0067] 1. Soil sample for test

[0068] The topsoil (3-10cm) of the farmland was taken from the experimental field of the teaching farm of South China Agricultural University.

[0069] After the soil samples were retrieved, they were first placed in a cool and ventilated place to air dry naturally, after air drying, they were ground, passed through a 2mm sieve, and a certain amount of allethrin was dissolved in acetone, and then soaked in diatomaceous earth to completely absorb allethrin. The soaked diatomaceous earth was placed in a fume hood to dry, and it was mixed into the soil so that the final concentration of allethrin in the soil was 50 mg / kg. Take 500g of soil samples and culture them in a constant temperature and humidity incubator at 30°C, press 1.0×10 7 The inoculation amount of CFU / mL was inserted into the HLJ7 degrading bacteria suspension, and disti...

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Abstract

The invention discloses bacillus megatherium and application of the bacillus megatherium in degrading pyrethroid insecticides. The degrading strain is a bacillus megaterium strain HLJ7, the bacillus megaterium strain HLJ7 is preserved in the Guangdong province microbial strain preservation center on September 4, 2019, and a preservation number of the bacillus megaterium strain HLJ7 is GDMCC No: 60760. The invention discloses the degradation effect of bacillus megatherium on pyrethroid insecticides such as allethrin for the first time, and the efficient and rapid degradation strain HLJ7 is obtained through screening. Based on the method, the bacillus megaterium has a remarkable biodegradation effect on pyrethroid insecticides such as allethrin, is high in degradation efficiency and stable in degradation performance, can tolerate high-concentration pesticides, and can be used for bioremediation of polluted environments such as water bodies and soil polluted by the pyrethroid insecticidessuch as allethrin. The invention provides a new development approach for breaking through the bottleneck of the existing treatment of pesticide residue pollution, enriches the germplasm resource library of pesticide degrading bacteria, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of pesticide pollution control. More specifically, it relates to a Bacillus megalosporus strain HLJ7 that degrades pyrethroid pesticides such as allethrin and its production and application. Background technique [0002] Pyrethroid pesticides include allethrin, etc. Allethrin is a synthetic type I pyrethroid formed by the esterification of D-trans first chrysanthemum acid and allyl alcohol ketone, because of its contact killing, repellent Most mosquito coils, aerosols, liquid sprays, etc. use this as a raw material. Although some insects have gradually developed resistance to this insecticide, it is still effective in controlling insect-borne diseases. Communication plays a vital role. With the continuous expansion of the scope of use of allethrin and the continuous increase of the dosage, there are also more and more reports about its toxicology and the pollution impact of residues on the environment. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02C12R1/11C02F101/38C02F101/36C02F101/34A62D101/04A62D101/22A62D101/26A62D101/28
CPCA62D3/02A62D2101/04A62D2101/22A62D2101/26A62D2101/28B09C1/10C02F3/34C02F2101/306C02F2101/34C02F2101/36C02F2101/38C12N1/205C12R2001/11
Inventor 陈少华李绮婷陈珊珊冯彦媚周天浩方加
Owner SOUTH CHINA AGRI UNIV
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