Holographic microscopic imaging device

Abstract

The invention discloses a holographic microscopic imaging implementation device. The device comprises an inverted microscope and a holographic imaging device, wherein the inverted microscope is provided with a light path inlet and a light path outlet; an object to be observed is illuminated by a linear polarization laser bright field, light carrying information of the object to be observed entersthe inverted microscope through the light path inlet, a holographic image amplified by the object is obtained through the inverted microscope, and the light path outlet of the inverted microscope is connected with the light path inlet of the holographic imaging device. The holographic imaging device is provided with a light path inlet and a light path outlet, and is used for transverse mode and polarization coupling of a light field and composite measurement. The quantitative phase imaging implementation device provided by the invention uses an imaging method of an unmarked specimen, does notgenerate photobleaching of a molecular structure due to light absorption caused by fluorescence imaging, overcomes the problem of toxicity of exciting light to biological cells, and can be used for observing evolution of living cells.

Description

technical field [0001] The invention relates to the technical fields of microscopic imaging technology, interference imaging and phase imaging, in particular to a holographic microscopic imaging device. Background technique [0002] When the light field passes through the inhomogeneous medium, it will interact with the medium and modulate the wavefront and amplitude of the light. This process is called light scattering. Generally, only elastic scattering is considered, that is, the wavelength of light does not change after scattering. Since the amplitude and phase of the scattered light carry information about the internal structure of the scattering object, the structure of the object can be recovered by measuring the scattered field. This process is called the solution of the inverse scattering problem. This is the basis for tomographic imaging of transparent biological tissue or cells. Since intracellular tissue has many highly dynamic structures that are difficult to s...

AI Technical Summary

Problems solved by technology

[0003] Imaging thin transparent samples with multiple scattering has always been a challenge in optical imaging. The difficulty lies in the fact that multiple scattering creates an incoherent background that ultimately leads to reduced image contrast, even for ordinary quantitative phase imaging.

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