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Composite nanoprobe with dumbbell structure as well as preparation method and application thereof

A nano-probe and dumbbell technology, which is applied in the field of composite nano-probe with dumbbell structure and its preparation, can solve the problems of low laser power and difficulty in effectively killing tumor cells, achieve good photostability, and enhance photothermal photodynamics effect of effect

Pending Publication Date: 2020-04-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the single photothermal therapy described in the above prior art is difficult to effectively kill tumor cells, the present invention provides a composite nanoprobe with a dumbbell structure, and the composite nanoprobe provided has enhanced photothermal and photodynamic effect, can effectively kill tumor cells at lower laser power, and ICG molecules under the protection of gold nanorods and mesoporous silica have good photostability

Method used

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  • Composite nanoprobe with dumbbell structure as well as preparation method and application thereof
  • Composite nanoprobe with dumbbell structure as well as preparation method and application thereof
  • Composite nanoprobe with dumbbell structure as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0046] (1) Preparation of gold nanorods

[0047] Soak all the containers with a strong oxidizing aqueous solution (10wt% potassium dichromate and 25wt% concentrated sulfuric acid), wash and dry them for use. Dissolve 0.364g CTAB into 8.34mL deionized water, then add 0.167mL 0.018mol / L chloroauric acid solution, add 1.16mL 0.01mol / L NaBH 4 Ice-water solution, stirred rapidly for two minutes, and placed in a water bath at 27° C. for 2 hours to obtain a gold nanorod seed solution.

[0048] Dissolve 1.82g CTAB into 45mL deionized water, then add 6mL chloroauric acid solution, then add 0.005mol / L AgNO 3 Solution 1.2mL and 0.4mL HCl (37%), add about 1.2 ~ 1.6mL 0.05mol / L ascorbic acid while stirring, at this time the solution turns from yellow to colorless, add 0.05mL gold nanorods prepared in the previous step during resting The seed solution (stirred gently for 10s) was placed in a water bath at 27°C for 12 hours, collected by centrifugation at 9000r / min for 20 minutes, and disp...

Embodiment 1

[0056] A compound nanoprobe of dumbbell structure, the preparation method is as follows:

[0057] 1) Load ICG: the obtained Au@mSiO 2 NDs particles were centrifugally and ultrasonically dispersed in 5 mL of indocyanine green (ICG 0.02 g) solution, and mixed in a flat-bottomed flask. Add a stirrer and stir at high speed for 30 minutes, take out the stirrer, place it in an ultrasonic water bath for ultrasonication, set the power to 100%, after ultrasonication for 12 hours, centrifuge to remove excess ICG molecules, wash twice with water and alcohol to obtain ICG-loaded Au @mSiO 2 NDs, dispersed in alcohol.

[0058] 2) Modification of PEG-FA: the prepared ICG-loaded Au@mSiO 2 After centrifugation, the NDs were ultrasonically dispersed in 50 mL of ethanol solution, added 0.5 mL of 5% (volume ratio) 3-aminopropyltriethoxysilane (APTES) ethanol solution, refluxed at 60°C for 12 hours, and centrifuged to make the surface The amino group was attached, washed twice with ethanol to ...

Embodiment 2

[0060] The difference from Example 1 is that the loading of indocyanine green in the prepared composite nanoprobe is 5% by changing the added amount of ICG; other compositions and steps are the same as in Example 1.

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Abstract

The invention discloses a composite nanoprobe with a dumbbell structure as well as a preparation method and application of the composite nanoprobe. The composite nanoprobe comprises a gold nanorod, solid silicon dioxide wrapping the two ends of the gold nanorod, mesoporous silicon dioxide wrapping the solid silicon dioxide and indocyanine green loaded in the mesoporous silicon dioxide; the gold nanorod, and the solid silicon dioxide and the mesoporous silicon dioxide which are positioned at two ends of the gold nanorod can form the dumbbell structure. The composite nanoprobe has the advantagesthat under the stimulation of near-infrared light the enhanced local electric fields at the two ends of the gold nanorod stimulate ICG molecules in the mesoporous silicon dioxide to continuously generate singlet oxygen, and meanwhile, the enhanced photo-thermal and photodynamic effects are finally obtained by utilizing the double photo-thermal effects of the gold nanorod and ICG, so that tumor cells can be effectively killed under lower laser power. In addition, ICG molecules under the protection of the gold nanorods and the mesoporous silicon dioxide do not generate photobleaching, and goodphotostability is achieved.

Description

technical field [0001] The invention relates to the technical field of nanobiological materials, and more specifically, to a dumbbell-structured composite nanoprobe and its preparation method and application. Background technique [0002] Photothermal therapy, as a means of physical therapy, has received increasing attention in tumor therapeutics. Through the method of photothermal heating, the environment of tumor cells is changed, and the tumor cells are degenerated and necrotic, so as to achieve the purpose of treatment. [0003] Nanogold nanorods have extremely high light absorption ability and good photothermal conversion ability, and are considered to be one of the most typical and potential photothermal reagents. It can effectively absorb near-infrared light and convert it into heat, thereby killing tumor cells. However, single photothermal therapy faces side effects such as low thermal signal, which prevents tumor cells from being completely killed, and excessive t...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/00A61K47/04A61P35/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K9/0009A61K41/0038A61K41/0052A61K41/0057A61K47/02A61P35/00B82Y20/00B82Y30/00B82Y40/00A61K2300/00
Inventor 殷金昌邵元智吴昊南汪项张玉田丽
Owner SUN YAT SEN UNIV
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