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Compound microbial inoculum and preparation method and application thereof

A technology of composite bacterial agents and strains, applied in the direction of biochemical equipment and methods, separation methods, chemical instruments and methods, etc., can solve problems such as the impact of the surrounding environment, the health damage of chemical plant employees and surrounding residents, and ensure the richness , high-efficiency degradation ability, and the effect of improving degradation efficiency

Inactive Publication Date: 2020-04-07
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is not handled properly, it will not only have a great impact on the surrounding environment, but also cause great damage to the health of the employees of the chemical plant and the surrounding residents.

Method used

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  • Compound microbial inoculum and preparation method and application thereof
  • Compound microbial inoculum and preparation method and application thereof
  • Compound microbial inoculum and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of composite bacterial agent, comprising the following steps:

[0035] (A) Domestication of the original bacterial liquid: collect soil samples and sewage samples in the oil pollution source, mix them, and inoculate them in sterile water to cultivate the original bacterial liquid, pass petrochemical waste gas into the original bacterial liquid for domestication, and obtain domesticated strains;

[0036] (B) single fermentation of main strains: screen out Klebsiella from the domesticated strains, add culture medium solution in the main fermenter, and inoculate the screened Klebsiella, carry out fermentation and culture to obtain main seed liquid;

[0037] (C) mixed fermentation of auxiliary strains: add directional culture medium solution in the secondary fermenter, and inoculate domesticated strains, carry out fermentation and culture to obtain auxiliary strain liquid;

[0038] (D) stirring and mixing the main strain liquid and the auxiliary strain...

Embodiment 2

[0049] A preparation method of composite bacterial agent, comprising the following steps:

[0050] (A) Domestication of the original bacterial liquid: collect soil samples and sewage samples in the oil pollution source, mix them, and inoculate them in sterile water to cultivate the original bacterial liquid, pass petrochemical waste gas into the original bacterial liquid for domestication, and obtain domesticated strains;

[0051] (B) Fermentation of main strains: Klebsiella is screened out from the domesticated strains, culture medium solution is added to the main fermenter, and the screened Klebsiella is inoculated, and the main strains are obtained by fermentation. Seed liquid;

[0052] (C) mixed fermentation of auxiliary strains: add directional culture medium solution in the secondary fermenter, and inoculate domesticated strains, carry out fermentation and culture to obtain auxiliary strain liquid;

[0053] (D) stirring and mixing the main strain liquid and the auxiliar...

Embodiment 3

[0064] A preparation method of composite bacterial agent, comprising the following steps:

[0065] (A) Acclimatization of the original bacterial liquid: collect soil samples and sewage samples in the oil pollution source, mix them, inoculate them in sterile water to cultivate the original bacterial liquid, pass petrochemical waste gas into the original bacterial liquid for domestication, and obtain domesticated strains;

[0066] (B) Fermentation of main strains: Klebsiella is screened out from the domesticated strains, culture medium solution is added to the main fermenter, and the screened Klebsiella is inoculated, and the main strains are obtained by fermentation. Seed liquid;

[0067] (C) mixed fermentation of auxiliary strains: add directional culture medium solution in the secondary fermenter, and inoculate domesticated strains, carry out fermentation and culture to obtain auxiliary strain liquid;

[0068] (D) stirring and mixing the main strain liquid and the auxiliary ...

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Abstract

The invention relates to the technical field of waste gas purification, in particular to a compound microbial inoculum and a preparation method and application thereof. The preparation method comprises the following steps: (A) carrying out domestication of an original bacterial liquid; (B) carrying out fermentation of a main strain; (C) carrying out mixed fermentation of auxiliary strains; (D) stirring and mixing the main strain liquid and the auxiliary strain liquid, centrifuging, and taking a supernatant to obtain microbial sludge; (E) stirring and mixing the microbial sludge and a drying protective agent, and drying to obtain the compound bacterial inoculums of petrochemical waste gas treatment. According to the preparation method of the compound microbial inoculum of the invention, strains are collected and domesticated in petroleum pollution sources, the main strain is subjected to single fermentation, the strains are subjected to directional mixed fermentation, the bacteria liquids of single fermentation and mixed fermentation are mixed, centrifugation is carried out to obtain the microbial sludge, and the microbial sludge is mixed with the drying protective agent to preparethe compound bacterial inoculums of petrochemical waste gas treatment. The production cycle is short, and the prepared microbial inoculum has high-efficiency degradation capability.

Description

technical field [0001] The invention relates to the technical field of exhaust gas purification, in particular to a composite bacterial agent and its preparation method and application. Background technique [0002] The petrochemical industry will produce a large amount of waste gas during the production process. The chemical waste gas has a strong odor and is very harmful to the human body. If it is not handled properly, it will not only have a great impact on the surrounding environment, but also cause great damage to the health of the employees of the chemical plant and the surrounding residents. [0003] Nowadays, the methods of treating chemical waste gas mainly include adsorption absorption method, catalytic oxidation method and biological treatment method. Due to the use of physical and chemical methods such as adsorption and absorption methods and catalytic oxidation methods are not ideal in the treatment of petroleum hydrocarbon waste gas and are likely to cause se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36B01D53/84C12R1/22
CPCB01D53/84B01D2251/95C12N1/20C12N1/36Y02A50/20
Inventor 赖柏民杨秋婵张保安
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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