Monocyte source exosome preparation applied to osteogenic differentiation of mesenchymal stem cells

Abstract

The invention relates to a monocyte source exosome preparation applied to osteogenic differentiation of mesenchymal stem cells. The monocyte source exosome preparation comprises exosomes secreted by monocytes in human peripheral blood and PBS (Phosphate Buffer Solution); a preparation method comprises the following steps: culturing monocytes sorted in the human peripheral blood at 37 DEG C until the monocytes grow to about 80%; the replacing with a complete medium without exosomes fetal bovine serum, and collecting the culture medium after culturing for 48 h; carrying out centrifugal treatmenton the culture medium; and storing 1xPBS resuspended sediment at -80 DEG C, so as to obtain the monocyte source exosome preparation. In view of a pathophysiological progress involving orthopedic diseases, the exosome preparation prepared from the exosomes secreted by the monocytes in the human peripheral blood has the properties of very good biocompatibility, no toxicity and no immune response, and can replace traditional osteogenic inducers, so that unknown risks caused by in-vivo injection are avoided and a novel treatment application is provided for bone defect, osteonecrosis, osteoporosis, bone metabolic diseases and the like on clinic.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a technology for preparing exosome preparations from peripheral blood mononuclear cells, in particular to an application of monocyte exosomes to promote osteogenic differentiation of mesenchymal stem cells. Background technique [0002] Bone tissue defect is caused by factors such as trauma, infection, tumor, and abnormal bone development, which cause the body to lose some bone mass and form a large gap. This problem is relatively common in clinical practice and difficult to deal with. How to promote the repair of bone tissue defects has always been the focus of research by researchers in this field. Traditional bone defect repair methods mainly include autologous bone grafting, allogeneic bone grafting, and artificial biomaterial replacement. But each has its own advantages and disadvantages. Autologous bone sources are limited, allogeneic and xenogeneic bone grafts have certain immun...

AI Technical Summary

Problems solved by technology

Autologous bone sources are limited, allogeneic and xenogeneic bone grafts have certain immunogenicity, which will lead to immune rejection, and there is also the risk of potential transmission of infectious diseases. Simple biomaterial filling often only has osteoconduction and lacks osteoinductive activity. Not suitable for large bone defects

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