Hyperspectral image based in-vivo tissue optical parameter measuring device and method

Abstract

The invention discloses a hyperspectral image based in-vivo tissue optical parameter measuring device and method. The device comprises a halogen lamp as a light source, a focusing lens, a light filtering wheel group, a camera and a spectrograph; the halogen lamp has the continuous broad-spectrum characteristic and is suitable for sample measurement in a visible spectrum; the light filtering wheelgroup is used as a light splitting device and comprises optical filters with different center wavelengths; the camera is matched with the focusing lens to realize high-resolution imaging; two-dimensional image information and a diffuse reflection spectrum of a phantom are captured, the acquisition time of the tissue imaging is shortened by collecting images with discrete wavelengths and utilizingWiener fitting principle based spectral reconstruction, and acquisition of multispectral images of different wavebands is realized. The method comprises the following steps of acquiring 8-channel images of a sample through the in-vivo tissue optical parameter measuring device; inputting a reflection value of a narrow-band light filtering channel and a measured diffuse reflection spectrum value, and performing reconstruction with a Wiener matrix to obtain a spectrogram; performing modeling and data fitting by combining a Monte Carlo look-up table method and a forward model to obtain a simulatedreflection spectrum of the sample; and acquiring tissue optical parameters and a phantom concentration by adopting an optimized deconstruction algorithm.

Description

technical field [0001] The invention relates to the field of optical imaging and detection of in vivo tissue, in particular to a device and method for measuring optical parameters of in vivo tissue based on hyperspectral images. Background technique [0002] Biological tissues are mostly composed of proteins, nucleic acids, carbohydrates, lipids, water, vitamins and trace elements. Because the macromolecular substances in the tissue have a strong scattering effect on photons, when the tissue structure and the content of various components in the tissue change, it will cause a corresponding change in the propagation path of light in the tissue, resulting in changes in the optical properties of the tissue. Variety. In biomedical photonics, the interaction between light and biological tissues is mainly in the form of absorption and scattering. The parameters such as absorption coefficient, scattering coefficient, refractive index and anisotropy factor are commonly used to quan...

AI Technical Summary

Problems solved by technology

However, the current method for extracting tissue optical parameters based on hyperspectral measurement is limited in terms of spectral imaging speed and requires multiple continuous spectral measurements. The system's spectral method is relatively complicated, and it is difficult to achieve fast imaging

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