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Casting method for preparing sphagnum sample for scanning electron microscope

A scanning electron microscope and sphagnum moss technology, which is applied in the direction of material analysis, measuring device, and instrument using wave/particle radiation, can solve the problems of difficult peeling of polymer resin, affecting the understanding of the internal structure of bryophytes, etc., and achieves easy operation and benefits. Development and application, the effect of simple operation

Active Publication Date: 2020-03-27
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing domestic and foreign research status on the application of resin casting method is more inclined to study the structural casting of human organs such as human pipes or woody plants, and there are few extensive researches on the structural casting of herbaceous plants. The biomass of herbaceous plants, especially bryophytes is small, and the polymeric resin formed during the casting process is difficult to peel off, which seriously affects the further understanding of the internal structure of bryophytes

Method used

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  • Casting method for preparing sphagnum sample for scanning electron microscope
  • Casting method for preparing sphagnum sample for scanning electron microscope
  • Casting method for preparing sphagnum sample for scanning electron microscope

Examples

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Effect test

Embodiment 1

[0041] A casting method for preparing a peat moss sample for scanning electron microscopy, comprising the following steps:

[0042] Step 1, take materials:

[0043] Choose fresh sphagnum moss blade as sample, put into the test tube with cover close to its height;

[0044] Step 2, fix:

[0045] Add 2.5v% glutaraldehyde fixative solution prepared by 0.1mol / L phosphate buffer solution with a pH of 6.8 to the test tube containing the sample, and fix the sample for 4h;

[0046] Step 3, rinsing and dehydration:

[0047] Add 0.1mol / L phosphate buffer solution with a pH of 6.8 to the fixed sample test tube, and replace the phosphate buffer solution repeatedly. In this embodiment, replace the phosphate buffer solution 3 times and rinse for 1.5 hours each time; Concentrations of 50%, 70%, 80%, and 90% ethanol dehydrating agents were used for dehydration at room temperature for 20 minutes at each concentration, and then dehydration was performed three times at room temperature using e...

Embodiment 2

[0063] A casting method for preparing a peat moss sample for scanning electron microscopy, comprising the following steps:

[0064] Step 1, take materials:

[0065] Choose dried sphagnum moss leaves as samples, and put them in a test tube with a lid close to its height;

[0066] Step 2. Resin casting:

[0067] Use a clean straw to absorb 1 wt% benzoyl peroxide-containing styrene solution containing twice the weight of water absorbed by the sample, and add it into the test tube containing the sample along the wall of the test tube, and do not touch the sample above the liquid level when adding , capped, placed vertically in a drying oven at 60°C, and polymerized for 4 days; wherein, the weight of water absorbed by the sample is the product of the dry weight of peat moss and the specific gravity of water absorption (times), and the specific gravity of water absorption (times)=(wet weight of peat moss -the dry weight of sphagnum moss) / the dry weight of sphagnum moss, the dry we...

Embodiment 3

[0079] A casting method for preparing peat moss for scanning electron microscopy, comprising the following steps:

[0080] Step 1, take materials:

[0081] Select the dried peat moss stem as a sample, and put it into a test tube with a lid close to its height;

[0082] Step 2. Resin casting:

[0083] Use a clean straw to absorb a styrene solution containing 1 wt% benzoyl peroxide that is twice the weight of the water absorbed by the sample, and add it into the test tube with the sample along the wall of the test tube, and do not touch the sample above the liquid level when adding , capped, placed vertically in a drying oven at 60°C, and polymerized for 4 days; wherein, the weight of water absorbed by the sample is the product of the dry weight of peat moss and the specific gravity of water absorption (times), and the specific gravity of water absorption (times)=(wet weight of peat moss -the dry weight of sphagnum moss) / the dry weight of sphagnum moss, the dry weight of sphag...

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Abstract

The invention discloses a casting method for preparing a sphagnum sample for a scanning electron microscope., relates to the field of preparation of herbaceous plant samples for scanning electron microscopes, and solves the problem that polymeric resin is difficult to peel off from sphagnum in the prior art. The method includes material taking, fixing, rinsing and dehydration, replacement and drying, resin casting, softening, corrosion and cleaning, gold plating and observation, thereby completing preparation of the sphagnum cast sample for the scanning electron microscope. The method has theadvantages that 1) the problem that the polymeric resin is difficult to peel off from sphagnum is solved; 2) in combination with the use of a scanning electron microscope, fine structures of cavities,transparent cells and green cells in the sphagnum moss can be known more stereoscopically, moisture and material transportation of the sphagnum moss can be further known, and subsequent development and application of the sphagnum moss are facilitated; and 3) the method is simple and easy to operate, low in technical cost and suitable for porous bryophytes and plants of which the taken materials can absorb and transport resin upwards.

Description

technical field [0001] The invention relates to the field of preparing herbal plant samples for scanning electron microscopy, in particular to a casting method for preparing peat moss samples for scanning electron microscopy. Background technique [0002] Sphagnum moss grows in swampy areas or forest depressions. Its plant cells have a very good water storage function, and it is gray-white in clusters in a dry state. Compared with absorbent cotton, its water absorption is even stronger. Due to the disinfection effect of sphagnum moss, after it has been sterilized, it can replace absorbent cotton as a material for making first aid kits and dressings. In addition, peat moss is widely used in real life, including medicine and health, packaging and transportation, environmental protection, energy development, and agrobiology. Peat moss belongs to the Bryophyte Sphagnum family. It is one of the important charcoal-making plants. Its total fixed carbon production accounts for half...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/2202
CPCG01N23/2202
Inventor 卜贵军汪正祥邸慧慧李政骋李盛强吴林
Owner HUBEI UNIV FOR NATITIES
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