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Preparation method for mesenchymal stem cell derived solution and applications thereof in cosmetics

A technology of mesenchymal stem cells and derivative solution, which is applied in the field of preparation of mesenchymal stem cell derivative solution, can solve the problems that cosmetics cannot be put on the market, consumers are difficult to identify, and it is difficult to penetrate the skin, etc., so as to enhance the barrier protection effect and increase the skin epidermis. Thickness, the effect of promoting division and proliferation

Inactive Publication Date: 2020-03-27
上海掘谜生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Modern women attach great importance to skin care in daily life and always look forward to finding safe and effective skin care products. However, the quality of skin care products on the market is uneven, making it difficult for consumers to identify
Some skin care products contain substances such as lead and mercury, ethanol, coloring agents and mineral oil, which are not only harmful to the skin, but may also cause serious dermatitis and infection.
[0004] With the rapid development of biomedicine, scientists have discovered that the culture supernatant of mesenchymal stem cells contains many active substances that can rejuvenate aging cells. However, due to technical barriers and production capacity problems, cosmetics containing biologically active substances cannot be put into the market. market
The cosmetics currently on the market are all directly extracted from plant components or processed by fine chemical production processes. The main active ingredients in the products are macromolecular compounds, which are not easy to penetrate into the skin, and cannot fundamentally improve the skin aging problem. It may also cause some adverse reactions such as allergies

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  • Preparation method for mesenchymal stem cell derived solution and applications thereof in cosmetics
  • Preparation method for mesenchymal stem cell derived solution and applications thereof in cosmetics
  • Preparation method for mesenchymal stem cell derived solution and applications thereof in cosmetics

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preparation example Construction

[0029] A preparation method of mesenchymal stem cell-derived liquid and its application in cosmetics, said method comprising the steps of:

[0030] (1) Wash the umbilical cord tissue 5 times with normal saline containing penicillin-streptomycin;

[0031] (2) Cut the umbilical cord tissue into small pieces of 1 cm with surgical scissors;

[0032] (3) Spread a 1 cm2 small piece of tissue with the dermal fat layer facing down in a petri dish, add 0.5 mg / mL collagenase IV to submerge the tissue piece, and place it in a refrigerator at 4°C for 16 hours to digest;

[0033] (4) Remove the epidermis, cut the tissue into small pieces of 1 mm3 with ophthalmic scissors, inoculate them into a culture bottle, and culture them with an appropriate amount of medium to obtain umbilical cord tissue-derived mesenchymal stem cells with a high survival rate. The culture medium is produced by American ThermoScientific Company and the product name is StemPro TM Medium for MSC SFM XenoFree;

[003...

Embodiment 1

[0039] (1) Collect the culture supernatant of the third passage mesenchymal stem cells.

[0040] (2) Add 3mg / L glutathione, 2mg / L coenzyme Q10, 3mg / L chitosan to the 1L mesenchymal stem cell culture supernatant collected in step (1); 2mg / L seaweed collagen and The volume ratio is 10% sodium hyaluronate, and the mesenchymal stem cell-derived liquid is obtained.

Embodiment 2

[0042] (1) The culture supernatant of passage 5 mesenchymal stem cells was collected.

[0043] (2) Add 6mg / L glutathione, 5mg / L coenzyme Q10, 6mg / L chitosan to the 1L mesenchymal stem cell culture supernatant collected in step (1); 5mg / L seaweed collagen and The volume ratio is 15% sodium hyaluronate, and the mesenchymal stem cell-derived liquid is obtained.

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Abstract

The invention discloses a preparation method for a mesenchymal stem cell derived solution and applications thereof in cosmetics. The preparation method includes the following steps: (1) using a mesenchymal stem cell medium to perform cultivation on mesenchymal stem cells from umbilical cord tissue, and collecting cell culture supernatant; and (2) adding glutathione, coenzyme Q10, chitosan, seaweedcollagen and sodium hyaluronate into the cell culture fluid obtained by the step (1) so as to obtain the mesenchymal stem cell derived solution. The prepared mesenchymal stem cell derived solution can be used for cosmetics, has deep moisturizing functions, can significantly increase the brightness and smoothness of skins, can effectively improve symptoms of slack skins, dullness and chloasma, andhas excellent repairing effects on injured skins.

Description

technical field [0001] The present invention relates to a kind of skin care product and its preparation method and application, in particular to a preparation method of mesenchymal stem cell derived liquid and its application in the field of cosmetics. Background technique [0002] The human life cycle is usually divided into three stages: growth period, maturity period and aging period. Under normal circumstances, when the age reaches 25 years old, various aging mechanisms of the body start to start, and various indicators related to aging also begin to decline, and people gradually enter the state of aging. Skin aging is one of the most noticeable features of human aging. With the change of age, the skin will gradually become dull, pigmentation, wrinkles, and sagging. These symptoms seriously affect the quality of life and mental health of modern women. "Resisting aging" has become a part of their life. an essential topic in . [0003] Modern women attach great importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/73A61K8/65A61K8/64A61K8/35A61Q19/00A61Q19/08A61Q19/02
CPCA61K8/982A61K8/736A61K8/735A61K8/65A61K8/64A61K8/355A61Q19/00A61Q19/08A61Q19/02A61K2800/30A61K2800/592
Inventor 王振纲吴利
Owner 上海掘谜生物科技有限公司
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