Surface plasma resonance biochemical analyzer integrated with nucleic acid amplification reaction

Abstract

The invention discloses a surface plasmon resonance biochemical analyzer integrated with nucleic acid amplification reaction. The surface plasmon resonance biochemical analyzer integrated with nucleicacid amplification reaction is driven by only one motor to achieve SPR angle automatic adjustment function, angles of incident light and a light receiving device can be simultaneously changed between21.5 degrees and 75.5 degrees, and the angle range can be further expanded. The prism coupling type SPR technology is combined with nucleic acid PCR amplification or isothermal amplification or otheramplification technologies, and the two functions of nucleic acid amplification and SPR detection are achieved on one sensing chip; and a PCR amplification temperature control device is integrated with a SPR prism coupling optical detection device; a sensing chip and a micro-fluidic flow cell of a flow system adopt a separable design, so that biomolecules are fixed on the chip by an offline method and an online method. Corresponding nucleic acid amplification products are detected by adopting a prism coupling type SPR optical system, and fluorescence labeling of the amplification products isnot needed. The SPR detection sensitivity is high, so that the nucleic acid molecule amplification period is shortened, the traditional nucleic acid amplification time is shortened, and the detectionspeed is high.

Description

technical field [0001] The invention relates to a plasma resonance biochemical analyzer, which belongs to the technical field of polymerase chain reaction (PCR); in particular, it relates to a surface plasmon resonance biochemical analyzer integrating nucleic acid amplification reaction. The invention can simultaneously perform PCR reaction and The surface plasmon resonance (Surface Plasmon Resonance, SPR) reaction can also perform PCR or SPR alone. Application scenarios can study the interaction between biomolecules, such as molecular recognition, immune regulation, immunoassay, rapid detection of toxins and antibiotics, nucleic acid detection, proteomic drugs and screening, and real-time detection of related pharmacokinetics. Background technique [0002] SPR technology is a modern, advanced and general-purpose label-free biochemical detection technology developed in recent years. This technology is a biochemical detection technology based on physical optical phenomena, us...

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Problems solved by technology

Compared with PCR technology, isothermal amplification has the advantages of high specificity, fast analysis speed, low cost, no need for heating and cooling process, and low risk. However, the current domestic isothermal amplification technology is not very mature and needs further development.

[0007] Whether it is the traditional PCR amplification method or the emerging isothermal amplification or other amplification methods, the detection of corresponding amplification products usually uses gel electrophoresis detection or fluorescence quantitative detection methods. These traditional detection methods usually need to be detected Samples are marked, and there are problems such as complicated operation, easy pollution and high cost

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