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Method for improving glutamic acid fermentation conversion rate

A technology of glutamic acid and conversion rate is applied in the field of improving the conversion rate of glutamic acid fermentation to achieve the effects of ensuring dissolved oxygen in fermentation, reducing fermentation volume, and promoting the release to extracellular space

Active Publication Date: 2020-03-24
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned process is a small-scale test in the laboratory stage, and it needs to be further optimized to verify the fermentation situation in the pilot test

Method used

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  • Method for improving glutamic acid fermentation conversion rate
  • Method for improving glutamic acid fermentation conversion rate
  • Method for improving glutamic acid fermentation conversion rate

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Experimental program
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Effect test

Embodiment 1

[0031] A method for improving the conversion rate of glutamic acid fermentation, comprising the steps of:

[0032] Brevibacterium flavum GDK-9 seed solution (OD 600nm 12) Insert 8% inoculum size into a 1000L fermenter equipped with 500L fermentation medium A for fermentation and cultivation, ferment and cultivate for 18h, then add 100L of fermentation medium B, continue to ferment and cultivate for 30h, and collect the fermented liquid; During the process, the fermentation temperature was controlled at 35°C, the ventilation ratio was 1:0.9, and the stirring speed was 300r / min. The residual sugar was maintained at not less than 1.0% by feeding glucose with a mass percentage concentration of 65%, and the pH was controlled by feeding ammonia water to 6.5. Feed defoaming agent to defoam; when fermented to 30h, feed K with a concentration of 20g / L at a flow rate of 5ml / min 2 HPO 4 , 2h before the end of fermentation, stop feeding K 2 HPO 4 .

[0033] The preparation method of ...

Embodiment 2

[0036] A method for improving the conversion rate of glutamic acid fermentation, comprising the steps of:

[0037] Brevibacterium flavum GDK-9 seed solution (OD 600nm 12) Insert 8% inoculum into a 1000L fermenter equipped with 500L fermentation medium A to carry out fermentation culture, ferment and cultivate for 24 hours, then add 100L fermentation medium B, continue to ferment and cultivate for 24 hours, and collect the fermented liquid; the whole fermentation culture During the process, the fermentation temperature was controlled at 35°C, the ventilation ratio was 1:0.8, and the stirring speed was 300r / min. The residual sugar was maintained at not less than 1.0% by feeding glucose with a mass percentage concentration of 60%, and the pH was controlled by feeding ammonia water to 6.7. Feed defoaming agent to defoam; when fermented to 30h, feed K with a concentration of 15g / L at a flow rate of 5ml / min 2 HPO 4 , until the end of fermentation. The preparation method of descri...

Embodiment 3

[0044] 1. Based on Comparative Example 1, CeCl was studied 3 Effects of rare earth salts on cell concentration, glutamic acid content and sugar-acid conversion rate.

[0045] Set CeCl 3 The added concentration is 0, 2.5, 5, 10, 20, 40mg / L, with CeCl 3 With the increase of the addition amount, the cell concentration and glutamic acid content both increased. When the addition amount was 10mg / L, the cell concentration and glutamic acid content reached the peak value. showed a downward trend, but the sugar-acid conversion rate did not change significantly during the whole process; indicating that the low concentration of CeCl 3 Rare earth salts can promote the proliferation of strains and increase the activity of glutamate-related synthetases, thereby increasing the production of glutamate. However, too high a concentration will cause the strain to proliferate and die, and the production of glutamate will decrease accordingly.

[0046] 2. Set CeCl 3 The addition amount was 10m...

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Abstract

The invention belongs to the technical field of amino acid production, and discloses a method for improving the glutamic acid fermentation conversion rate. The method comprises the following steps: 1)inoculating a fermentation culture medium A with a Brevibacterium flavum seed liquid for producing glutamic acid, and carrying out fermentation culture for 18-24 h; 2) then adding a fermentation medium B, continuing to ferment and culture for 24 h or above, and collecting the obtained fermentation liquor, wherein the total fermentation time is controlled to be 48-50 h; and 3) feeding an aqueous K2HPO4 solution with the concentration of 15-25 g / L at the flow rate of 5 ml / min when fermentation is performed for 30 h, and stopping the feeding before the fermentation is finished. The culture medium and the process are optimized to improve the conversion rate.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a method for improving the conversion rate of glutamic acid fermentation. Background technique [0002] Glutamic acid fermentation production is a biochemical process in which glutamic acid-producing bacteria decompose nutrients and synthesize required products, glutamic acid, in the course of their life activities. At present, the glutamic acid-producing bacteria used in industry include Corynebacterium glutamicum, Brevibacterium lactose fermenterum, Brevibacterium spirochete, Brevibacterium flavum, Brevibacterium ammoniaphagosus, etc. In the fermentation production process, there are many factors that affect the growth, reproduction, metabolism and synthesis of glutamic acid-producing bacteria. Through artificial intervention, these factors are purposely controlled to meet the metabolic and synthetic needs of glutamic acid-producing bacteria, which can...

Claims

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Application Information

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IPC IPC(8): C12P13/18C12N1/38C12R1/13
CPCC12N1/38C12P13/18
Inventor 刘世周赵兰坤孙钦波刘元涛李树标王小平赵杰
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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