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Stem cell composition and application thereof

A composition and stem cell technology, which is applied in the directions of drug combinations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., to achieve good treatment and relief effects, reduce treatment costs, and simplify the clinical treatment process.

Inactive Publication Date: 2020-03-24
青海晨菲制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While various treatments are effective, they also have their shortcomings

Method used

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  • Stem cell composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Obtaining of Deciduous Teeth Mesenchymal Stem Cells

[0041] Serum-free complete medium I: DMEM basal medium, 2mM / L L-glutamine, 4% Ultroser G solution, mix well, store in refrigerator at 4°C.

[0042] Serum-free complete medium II: containing DMEM basal medium, 2mM / L L-glutamine, 10% KnockOut SR serum replacement, 10ng / ml EGF, 10ng / ml bFGF.

[0043] Training method:

[0044] 1) Freshly exfoliated deciduous teeth were separated by Masako Miura et al. After digestion with type I collagenase, they were filtered and centrifuged to obtain mesenchymal stem cells for primary culture. The medium was 4.5ml serum-free complete medium II and 1.5ml serum-free The mixed medium of complete medium I, the culture period is 7-14 days;

[0045] 2) Discard the original cell culture medium in the T75 square flask cultured with P0 primary tooth mesenchymal stem cells, add 5ml DPBS to wash once;

[0046] 3) Add 0.8ml 0.25% trypsin to digest for 4 minutes, then resuspend the cells...

Embodiment 2

[0057] Example 2 Effect of stem cell composition on cellular gout model IL-1β

[0058] Studies have shown that gout is a typical autoinflammatory disease, and in the model of inducing gout in animals, it is accompanied by the release of various inflammatory factors, such as interleukin-6, interleukin-8, tumor necrosis factor-α and interleukin-1β (interleukin-1β, IL-1β), etc., wherein IL-1β is the main inflammatory factor produced in the gout model induced by monosodium urate (MSU). During the pathological development of gout, activated nuclear transcription factor-κB (NF-κB) can initiate and regulate the gene expression of many immune mediators and adhesion molecules related to inflammatory immune responses, and upregulate IL-1β, etc. The transcription of inflammatory cytokines produces more IL-1β, and IL-1β can reactivate NF-κB to generate a cascade reaction, causing continuous amplification of the inflammatory response and aggravating the disease.

[0059] (1) Isolation, cu...

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Abstract

The invention relates to a stem cell composition and an application thereof to gout diseases, in particular to a composition of mesenchymal stem cells from deciduous teeth and gallic acid, and an application of the composition to gout. The stem cell composition comprises the mesenchymal stem cells from deciduous teeth, the gallic acid and a stabilizing agent. A cell combination obtained through mixing the mesenchymal stem cells from deciduous teeth with the gallic acid has favorable treatment and relieving effects on the gout, and can reduce pain, arthrocele and the like in gout progress; andthe composition can fundamentally repair organism cell function and reduce the recurrence possibility of a patient.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a stem cell composition and its application in diseases, more specifically to the application of the stem cell composition in gout. Background technique: [0002] Stem cells are generally defined as clone-forming cells capable of self-renewal and multilineage differentiation. Stem cells can be divided into embryonic stem cells and adult stem cells according to their source. When a fertilized egg divides and develops into a blastocyst, the cells of the inner cell mass are called embryonic stem cells. Embryonic stem cells are totipotent, capable of self-renewal and differentiation into all tissues in the body, but their research and utilization involve certain ethical issues. Adult stem cells come from many tissues and organs of adult animals. Under certain conditions, adult stem cells can undergo a certain program of differentiation to form new functional cells, so that tissues and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/12A61P19/06A61K31/192
CPCA61K35/28A61K31/192A61K47/12A61P19/06A61K2300/00
Inventor 钟亮吴疆吴向阳
Owner 青海晨菲制药有限公司
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