Method for analyzing regulation effect of deer skin protein peptide on macrophage immune cells
A technology of macrophage regulation, applied in the field of biotechnology research and development analysis, can solve problems that have not yet been developed, and achieve the effect of fast detection speed, high sensitivity and low cost
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Embodiment 1
[0062] Take 40g of raw deerskin, shred it into small pieces about 0.5cm in length, take distilled water 10 times the quality of the raw deerskin, add it to the shredded raw deerskin, and under the condition of 4°C, Flash extraction was carried out 5 times, each time lasting 1 minute, at a speed of 3600 r / min, centrifuged for 10 minutes, and the supernatant was taken. The yield was 0.148%.
Embodiment 2
[0064] Take 40g of raw buckskin, shred it into small pieces about 0.5cm in length, take distilled water 20 times the quality of the raw buckskin, add it to the shredded raw buckskin, and under the condition of 4°C, Flash extraction was performed 5 times, each time lasting 1 minute, at a speed of 3600 r / min, centrifuged for 20 minutes, and the supernatant was taken. The yield was 0.286%.
Embodiment 3
[0066] Take 40g of raw deerskin, shred it into small pieces about 0.5cm in length, take distilled water 25 times the quality of the raw deerskin, add it to the shredded raw deerskin, and under the condition of 4°C, Flash extraction was carried out 8 times, each time lasting for 1 hour, at a speed of 3600 r / min, centrifuged for 30 minutes, and the supernatant was taken. The yield was 0.447%.
[0067] Example of research on the immune regulation of buckskin peptide on macrophage RAW264.7
[0068] Embodiment one, buckskin peptide protein content determination
[0069]Taking the bovine serum albumin mass concentration (X) as the abscissa and the absorbance OD value (Y) as the ordinate, draw a standard curve as figure 1 As shown, the protein content of the buckskin peptide solution calculated by the formula is 0.3660mg / mL respectively.
[0070] Example 2. Proliferation effect of different mass concentrations of DSPs on RAW264.7 macrophages
[0071] Both LPS and DSPs can promote...
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