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Chip device for cell migration experiment, preparation method thereof and experiment method

A cell migration and chip technology, which is applied in the field of chip devices for cell migration experiments, can solve the problems that the probability of cell contamination affects the experimental accuracy, and the process of cell arrangement is cumbersome. The effect of simple processing technology

Active Publication Date: 2020-03-06
HEFEI INNOVATION RES INST BEIHANG UNIV
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Problems solved by technology

[0005] A chip device, preparation method, and experimental method for cell migration experiments proposed by the present invention can solve the technical problems that the current related experiments use a cell arranger that is cumbersome and has a high probability of cell contamination that affects the accuracy of the experiment

Method used

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  • Chip device for cell migration experiment, preparation method thereof and experiment method
  • Chip device for cell migration experiment, preparation method thereof and experiment method
  • Chip device for cell migration experiment, preparation method thereof and experiment method

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Embodiment Construction

[0054] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments.

[0055] Such as figure 1 As shown, the chip device for cell migration experiments described in this embodiment, specifically the biochip, can be used for high-throughput cell migration research;

[0056] It includes a chip base layer, wherein the surface of the chip base layer contains multiple repeating functional units, and each functional unit contains a functional area for controlling cell migration and adhesion. The functional unit includes at least three functional areas: inoculation area, isolation area and migration area.

[0057]The seedin...

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Abstract

The invention relates to a chip device for cell migration experiment, a preparation method thereof and an experiment method, the technical problems that the process of using a cell arrangement deviceis tedious, the cell pollution probability is high, and the experiment precision is influenced can be solved. Wherein the surface of the chip comprises a plurality of repetitive function units; wherein each functional unit at least comprises three functional areas of an inoculation area, an isolation area and a migration area; the inoculation area is an area where cells are initially inoculated and adhered, and the surface of the inoculation area has hydrophobicity; the isolation area is an isolation column array arranged on the periphery of the inoculation area, the isolation column array surrounds the inoculation area to form an annular structure, a set gap is reserved between every two adjacent isolation columns, and the outer surfaces of the isolation columns have hydrophobicity; and the migration area is at periphery of the isolation area. The cell initial inoculation range is limited, cells can be controlled to migrate to the designated area range and direction, meanwhile, structures of different migration interfaces in vivo can be simulated, damage operation does not need to be conducted on the cells, and a chip does not need to be disassembled and assembled.

Description

technical field [0001] The invention relates to the technical field of cell migration analysis, in particular to a chip device, a preparation method and an experiment method for cell migration experiments. Background technique [0002] Cell migration is closely related to tumorigenesis and development, stem cell homing, embryogenesis, wound healing, inflammatory response, and host defense. Studying cell migration is of great significance for us to understand the principles behind these life activities and the treatment of related diseases. The traditional method for studying cell migration The scratch method is widely used in the traditional in vitro method for studying cell migration rate. This method does not divide the starting position of inoculation and the designated target migration area, but uses external force to remove a part of the adhered cells in the cell adhesion area after cell inoculation, resulting in an area without cell adhesion, and finally observes the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/00
CPCC12M23/02C12N5/00
Inventor 张冀聪王玄
Owner HEFEI INNOVATION RES INST BEIHANG UNIV
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