Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of PKP3 gene methylation in sperm activity detection agent and kit

A detection kit, sperm motility technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of accurate evaluation of sperm motility, less methylated regions, etc.

Pending Publication Date: 2020-02-28
深圳市龙华区人民医院
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are very few methylated regions related to sperm motility reported so far, and it is difficult to accurately evaluate sperm motility. Therefore, it is necessary to identify more methylated regions related to sperm motility, so as to provide a basis for the application of subsequent diagnosis and treatment. more basis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of PKP3 gene methylation in sperm activity detection agent and kit
  • Application of PKP3 gene methylation in sperm activity detection agent and kit
  • Application of PKP3 gene methylation in sperm activity detection agent and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0016] 1. Sperm separation

[0017] Seven asthenospermia volunteers (group A) and eight healthy volunteers (group N) were recruited, and sperm samples were obtained from the volunteers for genome-wide sequencing of methylation levels. Computer-assisted sperm analysis (CASA) was performed using a Sperm Class Analyzer, and sperm morphology was assessed by Diff-Quik rapid staining. Sperm cells with different vigor were separated by the following method: centrifuged at 300×g for 20 min to prepare discontinuous density gradient fractions (divided into 47.5, 57, 76 and 95% concentration layers), and from the 95% concentration layer (fraction a) High motility sperm cells were collected, and low motility sperm cells were c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of methylation level of a human PKP3 gene promoter region in preparing a detection agent for preparing sperm activity. The human PKP3 gene promoter region is a region corresponding to space from 392017th to 394717th of a human 11th chromosome. Analysis, research and verification on the basis of whole genome methylation sequencing results finds that the methylation level of the human PKP3 gene promoter region can serve as a molecular marker to detect the sperm activity, and when all methylation sites of the region are not completely methylated, it can be thought that activity of corresponding sperms is weak. The molecular marker can provide assistant evaluation information for diagnosis of asthenozoospermia and selection of sperms before artificial fertilization.

Description

technical field [0001] The invention relates to the field of human reproductive health, more particularly, relates to the application of the methylation level of the promoter region of the human PKP3 gene in the preparation of a detection agent for detecting sperm motility, and a detection kit for detecting sperm motility. Background technique [0002] More than 40% of infertile men were detected to suffer from asthenospermia. The etiology of asthenospermia is multifactorial, for example, it may be closely related to endocrine disorders, environmental factors, life experiences and even aging. Gene knockout and mutation studies in mice have shown that mutations in some genes are associated with altered sperm motility, but none of these genes have been shown to be associated with sperm motility in human studies. Although nearly 50% of infertility cases are believed to be caused by genetic defects, in some cases of male infertility, genetic factors do not appear to be the main...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154
Inventor 杜野桂耀庭邓琼马倩杨波
Owner 深圳市龙华区人民医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com