Method for preparing glutamic acid fermentation medium

A fermentation medium and glutamic acid technology, applied in the field of glutamic acid fermentation medium preparation, can solve the problems of unstable fermentation, separation and extraction, low nutritional value of hydrolysate, difficult control of fermentation process, etc. Conducive to microbial utilization, acid production, and stability of enzymatic hydrolysis products

Pending Publication Date: 2020-02-28
赵兰坤
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The optimization of the fermentation medium in the process of preparing glutamic acid by fermentation is an important factor to improve the fermentation efficiency. The applicant's previous patented technology "Method for preparing fermentation medium by using glutamic acid fermentation waste cells" has carried out an improvement on the existing medium Improvement and optimization, including 15% sorghum stalk hydrolyzate, 12% cell hydrolyzate, 5% glucose, 1.2% rice bran extract, 1% corn steep liquor, 0.02% shell powder, 0.02% ferrous sulfate heptahydrate, heptahydrate Magnesium sulfate is 0.02%, potassium dihydrogen phosphate is 0.01%, and the rest is water; this medium reduces the cost, but there are fermented nitrogen and carbon sources with more pigments, high viscosity and many impurities, which makes the fermentation process difficult to control and extremely It is easy to cause the instability of fermentation and the difficulty of separation and extraction; and the patent document uses hydrochloric acid in the treatment method of bacterial protein, which is easy to cause damage to amino acids, resulting in low nutritional value of hydrolyzed products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing glutamic acid fermentation medium
  • Method for preparing glutamic acid fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The enzymatic hydrolysis process of amino acid fermentation cells comprises the following steps:

[0034] The amino acid fermentation broth is separated by a disc separator with a rotation speed of 5000rpm and a centrifugation time of 3min to collect the bacterial precipitate; add a concentration of 5g / L potassium dihydrogen phosphate aqueous solution to the bacterial precipitate and configure it to a concentration of 200g / L For the bacterial cell suspension of L, use a high-pressure homogenizer to homogenize the bacterial cell suspension, the homogenization pressure is 90Mpa, the temperature is 25°C, homogenize 3 times; stop the homogenization treatment, centrifuge at 1000rpm for 5min, and collect The upper layer of liquid and sediment (cell wall fragments and macromolecular proteins), then add 10 times the weight of purified water to the sediment, stir evenly, and use 20kHz ultrasonic waves for 20 minutes, then adjust the temperature to 45 ° C, using Neutral protease ...

Embodiment 2

[0036] The enzymatic hydrolysis process of amino acid fermentation cells comprises the following steps:

[0037]The amino acid fermentation broth is separated by a disk separator with a rotation speed of 4000rpm and a centrifugation time of 3min to collect the bacterial precipitate; add a concentration of 7g / L potassium dihydrogen phosphate aqueous solution to the bacterial precipitate, and configure it to a concentration of 250g / L For the bacterial cell suspension of L, use a high-pressure homogenizer to homogenize the bacterial cell suspension. The homogenization pressure is 90Mpa, the temperature is 25°C, and the homogenization is performed 3 times; stop the homogenization treatment, centrifuge at 1000rpm for 4min, and collect The upper liquid and sediment (cell wall fragments and macromolecular protein), then add 8 times the weight of purified water, stir evenly, use 20kHz ultrasonic wave for 30min, then adjust the temperature to 45°C, and use neutral protease to hydrolyze ...

Embodiment 3

[0039] The enzymatic hydrolysis process of amino acid fermentation cells comprises the following steps:

[0040] The amino acid fermentation broth is separated by a disc separator with a rotation speed of 5000rpm and a centrifugation time of 3min to collect the bacterial precipitate; add a concentration of 10g / L potassium dihydrogen phosphate aqueous solution to the bacterial precipitate, and configure it to a concentration of 220g / L For the bacterial cell suspension of L, use a high-pressure homogenizer to homogenize the bacterial cell suspension, the homogenization pressure is 90Mpa, the temperature is 25°C, homogenize 3 times; stop the homogenization treatment, centrifuge at 1000rpm for 5min, and collect The upper liquid and sediment (cell wall fragments and macromolecular protein), then add 9 times the weight of purified water to the sediment, stir evenly, use 20kHz ultrasonic wave for 20min, and then adjust the temperature to 45 ℃, using Neutral protease hydrolysis, the a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of amino acid production, and discloses a method for preparing a glutamic acid fermentation medium, which comprises the steps of: taking fermentation medium materials, including glucose, bacterial protein extract, K2HPO4, MgSO4.7H2O, MnSO4.H2O, FeSO4.7H2O, VB1 and biotin; uniformly stirring the raw materials, adjusting the pH to 6.5, sterilizing at 121DEG C for 15 min, and naturally cooling to obtain the fermentation medium. According to the preparation method, the bacterial protein hydrolyzed extract is used for replacing yeast extract, so that the cost is greatly reduced, the added value of bacterial protein is improved, and the acid production efficiency is higher.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a preparation method of a glutamic acid fermentation medium. Background technique [0002] Glutamic acid fermentation waste bacteria is a by-product of the production process of MSG, and it is a single-cell protein isolated during the process of extracting and refining MSG, with an annual output of up to one million tons. In order to save resources, most of the monosodium glutamate manufacturers currently sell glutamic acid bacterial protein as feed. Although they have achieved certain economic benefits, their application value has not been fully tapped. Through the analysis of the nutritional components of the bacterial protein, it is found that the protein content is rich, the amino acid components are complete, and it is rich in vitamins, nucleic acids, polysaccharides, etc. Therefore, it is of great significance to use glutamic acid bacterial protei...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/18C12N1/06C12N1/20C12N13/00C12R1/15
CPCC12P13/18C12N1/066C12N1/20C12N13/00
Inventor 李德衡赵凤良翟德亮赵杰
Owner 赵兰坤
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap