Extraction and purification method of grape aromatic glycoside and library building, identification and quantification method thereof

Abstract

The invention discloses an extraction and purification method of a grape aromatic glycoside and a library building, identification and quantification method thereof. The extraction and purification method comprises the steps of selecting and peeling grape fruits, carrying out extraction, separation and purification of a terpene aromatic glycoside, building a terpene aromatic glycoside mass spectral library, performing LC-MS analysis of the terpene aromatic glycoside, using the terpene aromatic glycoside mass spectral library, and performing qualitative and quantitative analysis of the terpenearomatic glycoside. The extraction and purification method is low in cost and high in extraction efficiency, and the precision and reliability of research are guaranteed through system matching establishment of the method. The methods established by the research are simple and feasible in actual analysis, and have good popularization and application values.

Description

[0001] 1. Technical field: [0002] The invention relates to a chemical analysis method for natural aroma substances, in particular to a method for extracting and purifying grape aroma glycosides and a method for establishing a database, identifying and quantifying them. [0003] 2. Background technology: [0004] Terpene-like aroma glycosides are important aroma precursors in fruits, tea, flowers and their processed products. They have the potential to produce free terpene-like aroma substances by hydrolysis, so they have become a research hotspot at this stage. The typical aroma characteristics of wine depend on the release and hydrolysis of aroma precursors on grape skins during fermentation. Terpene aroma glycosides are one of the most important types. in accordance with. Due to the difficulty in the artificial synthesis of terpene-like aroma glycosides and the lack of standard products of terpene-like aroma glycosides on the market, the chemical structure of grape terpene...

AI Technical Summary

Problems solved by technology

Although there have been many reports on the application of LC-MS technology to the analysis of terpene-like aroma glycosides, the qualitative identification of related research still stays in the preparation of glycosides, the comparison of ion fragments by mass spectrometry, and the confirmation of aglycones. The types of terpene-like glycosides identified Restricted, there is no mass spectrometry library of terpene-like aroma glycosides that can be promoted, which severely limits the LC-MS identification and analysis of terpene-like aroma glycosides

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