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Peroxidase catalytic silver deposition-based method for detecting GPC3

An electrochemical and solution technology, applied in the field of detection of GPC3 based on electrochemical biosensors, can solve the problems of complexity, high cost, cumbersome operation, etc., and achieve the effect of simple operation, easy miniaturization, and sensitive detection

Active Publication Date: 2020-02-21
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

However, the method is cumbersome, complicated and expensive
There is no report on the construction of a rapid and portable method for the detection of GPC3 based on the GPC3 nanoaptamer sensor deposited by heme / graphene (Hemin / GO nanoparticles, HGNs) catalyzed by peroxidases

Method used

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  • Peroxidase catalytic silver deposition-based method for detecting GPC3
  • Peroxidase catalytic silver deposition-based method for detecting GPC3
  • Peroxidase catalytic silver deposition-based method for detecting GPC3

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Embodiment Construction

[0043] The present invention will be described in detail below in combination with specific embodiments.

[0044] figure 1 is a schematic of the detection of GPC3 based on peroxidase-catalyzed silver deposition. First, HGNs composites were prepared by a one-step reduction method, and HGNs were used to immobilize GPC3-Apt to form HGNs-Apt signaling probes. Using gold nanoparticles to modify activated SPCE, GPC3-Apt was immobilized on the surface of gold-deposited screen-printed electrodes by physical adsorption. When GPC3 was further modified, the specific recognition reaction between GPC3-Apt and GPC3 generated aptamer-antigen complexes arrayed on the electrode surface. HGNs-Apt nanomaterials were immobilized on the electrode surface through π-π bonds and electrostatic adsorption to form aptamer-antigen-aptamer sandwich complexes, and finally silver nanoparticles were deposited on SPCE. HGNs with peroxidase-like catalyzed deposition induce silver deposition. Generates a curr...

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Abstract

A peroxidase catalytic silver deposition-based method for detecting GPC3 comprises the steps of building an electrochemical nanometer sensor, activating and modifying a silk-screen printed electrode,building a biological sensing interface, drawing a working curve of the GPC3 and detecting a to-be-detected sample. An HGNs-Apt signal probe is fabricated by taking HGNs as a carrier, and an Apt-GPC3-HGNs-Apt sandwiched electrochemical nanometer aptamer sensor is built; silver ions in a solution are reduced to metal silver deposited on a surface of the electrode by H2O2 and by means of a catalyticeffect of peroxidase of HGNs, the deposition quantity of Ag is obtained according to quantity of GPC3 protein, and quantization is performed by differential pulse voltammetry (DPV); and current response of Ag and GPC3 concentration are in favorable positive correlation within a range being 10.0-100.0 micrograms per mL, and GPC3 detection is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting GPC3 based on an electrochemical biosensor. Background technique [0002] Glypican-3 ( GPC3 ) is a liver cancer marker. The main methods of GPC3 detection include ELISA, fluorescent immunoassay, enzyme-linked immunosorbent assay and so on. Immunological methods for measuring serum GPC3 have poor detection sensitivity and are difficult to apply clinically. The invention patent with the publication number CN 106645724A discloses a detection method for the surface marker molecule GPC3 of circulating tumor cells. By lysing red blood cells in the blood, all the remaining nucleated cells are tiled and enriched on the nano-substrate and fixed by using nanotechnology. All cells were labeled with nuclear fluorescent dye DAPI, all cells were incubated with GPC3 primary antibody, and then incubated with secondary antibody labeled with FITC f...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48G01N33/68G01N33/574
CPCG01N27/3278G01N27/48G01N33/68G01N33/57488
Inventor 李桂银赵乐李文湛陈敏梁晋涛周治德
Owner GUILIN UNIV OF ELECTRONIC TECH
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