Co-crosslinking immobilization method of beta-galactosidase

Abstract

The invention relates to a co-crosslinking immobilization method of beta-galactosidase. Oil-soluble diethylene glycol diacrylate is used as a cross-linking agent, a reactant in an aqueous phase is a molecule compound prepared from amino containing beta-galactosidase, aminated epoxy resin and beta-cyclodextrin, through a Michael addition reaction of dual bonds and amino, the co-crosslinking polyreaction is generated at low temperature, and the immobilization beta-galactosidase of different loading quantities can be prepared. The cross-linking degree is controlled, so that the dispersibility isimproved, and internal mass transfer microenvironment can be improved. An immobilized enzyme has high catalytic activity, and when the loading quantity is 56mg of enzymes / 1g of carriers, the relativeactivity can achieve the highest value, and is 93% of that of free enzymes.

Description

technical field [0001] The invention relates to the technical field of immobilized enzyme biocatalysis, in particular to a method for co-crosslinking and immobilizing β-galactosidase. The novel immobilized β-galactosidase can be specially used to improve the sweetness of dairy products. It can also reduce the content of lactose, and it is convenient for lactose intolerant consumers to accept milk, a natural and well-balanced food. Background technique [0002] β-galactosidase is an acidic hydrolase (isoelectric point 4.6), with a molecular weight of 540kD and a tetramer structure, widely present in animals, plants and microorganisms. Lactose is a unique sugar in milk. However, children and adults are prone to lack of lactase in their bodies, and drinking milk often causes indigestion and absorption of lactose. If a person with lactase deficiency consumes more lactose at one time, it will cause symptoms such as abdominal distension and diarrhea, which is called lactose into...

AI Technical Summary

Problems solved by technology

The most commonly used cross-linking agent is water-soluble glutaraldehyde, which has high reactivity and is difficult to control the dosage. It is easy to cause excessive cross-linking of the enzyme and cause a great loss of enzyme activity. In addition, the traditional cross-linking method often It is necessary to precipitate and aggregate enzyme molecules before cross-linking, which will not only cause waste of enzymes, but also block mass transfer channels, and cannot fully exert the catalytic efficiency of enzymes

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