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Signaling protein composition for controlling root-knot nematodes and preparation method and application of signaling protein composition

A root-knot nematode technology and a composition are applied to the signal protein-containing composition for preventing and treating root-knot nematode and the field of preparation thereof, which can solve the problem of drug resistance and microbial degradation, weaken the use effect of nematicides, reduce crop biomass and yield, and prevent The effect depends on the colonization ability and other issues to achieve the effect of efficient control of root-knot nematode disease, weakened infection ability, and difficult to drug resistance.

Active Publication Date: 2020-02-18
WEIFANG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, problems such as drug resistance and microbial degradation caused by the long-term frequent use of a single type of nematicide have weakened the effect of these nematicides
Driven by interests, farmers often choose to increase the use of pesticides, which results in increased production costs and substandard pesticide residues
At present, the auxiliary biological agents on the market are mostly through fungal predation and root probiotics, and their control effect is very dependent on their colonization ability, so the control effect is very unstable in different habitats.
At the same time, the use of common root-knot nematode disease control drugs itself often causes phytotoxicity of crops, reducing crop biomass and yield.

Method used

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  • Signaling protein composition for controlling root-knot nematodes and preparation method and application of signaling protein composition
  • Signaling protein composition for controlling root-knot nematodes and preparation method and application of signaling protein composition
  • Signaling protein composition for controlling root-knot nematodes and preparation method and application of signaling protein composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: the control of tomato root-knot nematode by wettable powder

[0062] Formula: 20% composition (0.65% HP, 13.0% SP, 6.35% IM), 2.5% nonylphenol polyoxyethylene ether (wetting agent), 2.5% sodium lignosulfonate (dispersing agent), 0.5% cyclo Oxygenated Soybean Oil (suspending agent and stabilizer), the balance is talc (filler). The above-mentioned raw materials are prepared through the process steps of mixing, ultrafine airflow pulverization and mixing. In addition, the 19.35% (13.0%SP, 6.35%IM) wettable powder formulation is different in that HP is not added, and the rest is the same. The active ingredient in the 5%HP WP contains only 5%HP, the active ingredient in the 19.2%IM WP contains only 19.2% IM, and the rest of the excipients and additives are the same. The 70.1% SP wettable powder contains only 70.1% of the fermentation product of Polychaeta figifera, and the rest of the auxiliary materials and adjuvants are the same.

[0063] This embodiment is...

Embodiment 2

[0067] Example 2 Control of cucumber root-knot nematode by 20% wettable powder

[0068] The formula is the same as above, and this embodiment is applied to the control of cucumber root-knot nematode. The experiment was carried out from June to July 2018 in a cucumber greenhouse in Shouguang City, Shandong Province, which has been used for many years and is seriously infected with root-knot nematode. The root-knot nematodes in this greenhouse are mainly root-knot nematodes incognita, and a small amount of root-knot nematodes in peanuts. The test method and design are the same as above, and the test object used is cucumber seedlings, and the variety is "Dongwei". After 30 days, the control effect was detected, and the detection items included the number of root knots, the area of ​​the second cucumber leaf (leaf area = leaf length × leaf width × 0.743), plant height, and cucumber yield. The specific results are shown in Table 4.

[0069] Table 4

[0070]

[0071] The resea...

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Abstract

The invention belongs to the field of agricultural plant protection and particularly relates to a signaling protein composition for controlling root-knot nematodes and a preparation method and application of the signaling protein composition. The composition comprises an active component A, namely signaling protein, an active component B, namely biocontrol spore powder and an active component C, namely non-fumigating nematicide. According to the root-knot nematode controlling method, biological control is used as a main means, and chemical control is used as an auxiliary means; by improving the crop stress resistance and the crop growing vitality, weakening the root-knot nematode infection capability and the after-infection fixed feeding site generation capability and the like, the purposeof controlling the root-knot nematodes through a united function is achieved; the components of the composition show an obvious synergetic effect within a certain matching range, the control effect is remarkably improved compared with separate use of the contained components, the dosage is reduced, and possible residues of a small quantity of synthetic chemical substances in the ecological environment are reduced.

Description

technical field [0001] The invention belongs to the field of agricultural plant protection, in particular to a signal protein-containing composition for controlling root-knot nematodes, a preparation method and application thereof. Background technique [0002] root-knot nematodes ( Meloidogyne spp.) is a soil-borne disease with a wide range of hosts and can infect almost all vascular plants, so most vegetable crops are good hosts for it. In the life cycle of root-knot nematodes, except for the second instar larvae in the infestation state, the other stages are in the roots of plants or in the egg shells. The first instar larvae are formed in the egg shell, and after a molt, the second instar larvae with a stylet are formed, and the second instar larvae use the stylet to pierce the egg shell and enter the soil. The second-instar larvae in the infested state swim in the water in the soil gap, look for a suitable host and infect the root at the root tip of the host, and stop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N37/46A01N57/32A01P5/00A01P21/00C12N1/14C12R1/645
CPCA01N37/46A01N57/32C12N1/14
Inventor 岳向国
Owner WEIFANG UNIV OF SCI & TECH
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