Single-pixel multispectral calculation imaging system and imaging method based on bundled optical fibers

A technology of computational imaging and fiber optic spectrometer, applied in the field of single-pixel multi-spectral computational imaging system, can solve the problems of high use conditions, high price, and huge control data flow

Active Publication Date: 2020-02-14
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

At present, the commonly used DMD frame frequency is about 100-300Hz, and there is also a DMD with a high frame frequency of 20KHz, but the price is high, the control data flow is huge, and the use conditions are relatively high.

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  • Single-pixel multispectral calculation imaging system and imaging method based on bundled optical fibers
  • Single-pixel multispectral calculation imaging system and imaging method based on bundled optical fibers
  • Single-pixel multispectral calculation imaging system and imaging method based on bundled optical fibers

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Embodiment Construction

[0035]The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0036] The frequency of DMD (digital micromirror) single frame flip is generally 100-200Hz, reconstructed through 5-10 pictures, and the common image frame frequency is 30-50Hz. The bandwidth of single-pixel sensors such as PMT (photomultiplier tube) or APD (avalanche diode) can reach 10KHz-100KHz, and this bandwidth range can also be reached after light source modulation. The current embodiment adopts 3KHz imaging and modulation rate, and the algorithm uses 64 Image reconstruction, frame rate up to 470Hz. This high-speed fluorescence imaging method has good application prospects in biological observation, flow cytometry and other fields.

[0037] Based on the above basis, the present invention uses a fiber optic spectrometer to replace point or array single photon detectors, which can realize continuous spectrum imaging.

[0038] Such as figure 1 As sho...

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Abstract

The invention provides a single-pixel multispectral calculation imaging system and imaging method based on the bundled optical fibers, thereby realizing the high anti-interference capacity, reducing hardware consumption resources, and reducing the requirements for texts. The invention provides a novel method for observing matrix changes by replacing a DMD (Digital Micromirror Device) with bundledoptical fibers and an image sensor (CCD). One of the bundled optical fibers is connected with a sensor to collect signals, and the other branches are connected with a modulatable light source. Different light intensity distribution is formed on an irradiated target in different on-off states of the light sources; according to the light intensity distribution, the observation matrix is able to realize modulation of 10 to 100KHz simply by the light source and thus the change frequency of the observation matrix can also reach the rate. After the system is built, he observation matrix is collectedand recorded frame by frame through a CCD and a recovery model is built; and no collection is performed in the follow-up actual observation process. With the provided method, the high-speed single-pixel imaging can be realized.

Description

technical field [0001] The invention relates to the technical field of optical computing imaging, in particular to a single-pixel multispectral computing imaging system and imaging method based on bundled optical fibers. Background technique [0002] The current high-sensitivity and rapid fluorescence detection technologies mainly include single-molecule detection technology, time-resolved technology and super-resolution measurement technology. Among the imaging technologies aimed at high temporal resolution, fluorescence lifetime imaging microscopy (FLIM) is currently a widely used technology in the field. However, the stability of the nano-displacement scanning platform included in it is poor, and the scanning process is complicated, which not only increases the manufacturing cost, but also greatly prolongs the testing time of nanomaterials and biomacromolecules, so the success rate is also significantly affected. Therefore, it is currently difficult to achieve high tempo...

Claims

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Application Information

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IPC IPC(8): G01J3/28G01J3/44G01J3/02G01J3/10G01N21/64G01N21/01
CPCG01J3/0218G01J3/10G01J3/2803G01J3/2823G01J3/4406G01J2003/102G01J2003/2826G01N21/01G01N21/6402G01N21/6456G01N2021/0112G01N2021/6419G01N2021/6484
Inventor 李晓琼樊云龙钱成杨春华冷坤
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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