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A molecular marker c7 resistant to Vibrio parahaemolyticus in portunus trituberculatus and its application

A technology of Portunus trituberculatus and molecular markers, applied in the direction of resistance to vector-borne diseases, measurement/inspection of microorganisms, biochemical equipment and methods, etc., to achieve the effects of accurate and reliable methods, reducing the probability of infection, and promoting healthy reproduction

Active Publication Date: 2021-06-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the molecular markers of Portunus trituberculatus resistance to Vibrio parahaemolyticus have not been reported. Therefore, the development of molecular markers resistant to Vibrio parahaemolyticus is of great significance for the healthy cultivation and breeding of Portunus trituberculatus

Method used

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  • A molecular marker c7 resistant to Vibrio parahaemolyticus in portunus trituberculatus and its application
  • A molecular marker c7 resistant to Vibrio parahaemolyticus in portunus trituberculatus and its application
  • A molecular marker c7 resistant to Vibrio parahaemolyticus in portunus trituberculatus and its application

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Embodiment 1

[0023] 1. Screening of candidate molecular markers related to traits of resistance to Vibrio parahaemolyticus

[0024] 1. Sequencing data filtering and comparison

[0025] The DNA extraction was carried out using the kit of Quanshijin Company, and the DNA extraction was carried out by using the principle of specific adsorption of DNA on the silica gel membrane spin column. First, put about 30mg of tissue samples into a 1.5ml sterile enzyme centrifuge tube, add 200μl LysisBuffer 8 (LB8) and 20μl RNaseA (10mg / ml), shake for about 10s, incubate at room temperature for 2min, add 20μl Proteinase K (20mg / ml), fully shake and mix, incubate at 55°C until completely lysed, add 1.5 times the volume of Binding Buffer 8 (BB8), mix well and add to the spin column, in a high-speed low-temperature refrigerated centrifuge (Eppendorf5804R) Centrifuge at 12000rpm for 30s, and discard the waste liquid. Then add 500μl of Clean Buffer 8 (CB8), centrifuge at 12000rpm for 30s, discard the waste so...

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Abstract

The invention provides a molecular marker C7 resistant to Vibrio parahaemolyticus in portunus trituberculatus and its application. The nucleotide sequence of the molecular marker C7 is shown in SEQ ID No.1, and the nucleotide sequences of the primer pair for detecting the molecular marker C7 are shown in SEQ ID No.2 and SEQ ID No.3. The molecular marker C7 is a SNP marker, and its resistance genotype of Vibrio parahaemolyticus is TT genotype. The molecular marker C7 provided by the present invention can be used to speed up the breeding of excellent traits of Portunus trituberculatus resistant to Vibrio parahaemolyticus, promote the breeding process of Portunus trituberculatus improved varieties, and is not limited by the growth stage, which is beneficial to the healthy cultivation of Portunus trituberculatus And sustainable development, so it has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers, in particular to a molecular marker C7 of Portunus trituberculatus resistant to Vibrio parahaemolyticus and an application thereof. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) belongs to Crustacea, Decapoda, Portunidae, Portunidae, commonly known as Portunus, and is an important large-scale marine economic crab in my country. Swimming crab meat is delicious and nutritious. It enjoys a high reputation at home and abroad and is deeply loved by consumers. Vibrio parahaemolyticus is a marine bacterium mainly derived from seafood such as fish, shrimp, crab, shellfish and seaweed. The bacterium has strong survival ability and can survive in seawater for 47 days. If humans eat seafood containing Vibrio parahaemolyticus, it will cause acute illness, abdominal pain, vomiting, diarrhea and other symptoms. The trait of resistance to Vibrio parahaemolytic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172Y02A50/30
Inventor 吕建建阎德平金玲刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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