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A low-salt-tolerant molecular marker c62 of Portunus trituberculatus and its application

A technology of Portunus trituratus and molecular markers, applied in the direction of microbial determination/inspection, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the lack of markers in molecular marker-assisted breeding, juvenile crab feeding rate, metamorphosis rate and Reduce the survival rate, affect the normal molting and growth of adult crabs, etc., to achieve the effect of promoting healthy reproduction, improving feeding rate, and increasing the number of

Active Publication Date: 2021-06-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Portunus trituberculatus adapts to a salinity of 20-35. However, because it is mainly cultured in outdoor ponds or tidal flats, the salinity of the cultured water often drops sharply due to factors such as heavy rains, which makes the feeding rate, metamorphosis rate and survival rate of young crabs Significantly decreased, it will also affect the normal molting and growth of adult crabs
At present, there are few studies on the development of low-salt tolerance molecular markers in Portunus trituberculatus, and the industry lacks markers that can be applied to molecular marker-assisted breeding

Method used

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  • A low-salt-tolerant molecular marker c62 of Portunus trituberculatus and its application
  • A low-salt-tolerant molecular marker c62 of Portunus trituberculatus and its application
  • A low-salt-tolerant molecular marker c62 of Portunus trituberculatus and its application

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Experimental program
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Embodiment 1

[0022] 1. Screening of candidate molecular markers related to low-salt tolerance traits

[0023] 1. Sequencing data filtering and comparison

[0024] The DNA extraction was carried out using the kit of Quanshijin Company, and the DNA extraction was carried out by using the principle of specific adsorption of DNA on the silica gel membrane spin column. First, put about 30mg of tissue samples into a 1.5ml sterile enzyme centrifuge tube, add 200μl LysisBuffer 8 (LB8) and 20μl RNaseA (10mg / ml), shake for about 10s, incubate at room temperature for 2min, add 20μl Proteinase K (20mg / ml), fully shake and mix, incubate at 55°C until completely lysed, add 1.5 times the volume of Binding Buffer 8 (BB8), mix well and add to the spin column, in a high-speed low-temperature refrigerated centrifuge (Eppendorf5804R) Centrifuge at 12000rpm for 30s, and discard the waste liquid. Then add 500μl of Clean Buffer 8 (CB8), centrifuge at 12000rpm for 30s, discard the waste solution (repeat once), ...

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Abstract

The invention provides a low-salt-tolerant molecular marker C62 of Portunus trituberculatus and its application. The nucleotide sequence of the molecular marker C62 is shown in SEQ ID No.1, and the nucleotide sequences of the primer pair for detecting the molecular marker C62 are shown in SEQ ID No.2 and SEQ ID No.3. The molecular marker C62 is a SNP marker, and its low-salt tolerance genotype is AG genotype. The molecular marker C62 provided by the present invention can not be limited by the growth stage of Portunus trituberculatus, and can be used to speed up the breeding process of Portunus trituberculatus and rapidly select crab species with excellent low-salt tolerance, which is beneficial to the health of Portunus trituberculatus Breeding and sustainable development, so it has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers, and in particular relates to a low-salt-tolerant molecular marker C62 of Portunus trituberculatus and an application thereof. Background technique [0002] Portunus trituberculatus (Portunus trituberculatus) belongs to Crustacea, Decapoda, Portunidae, Portunidae, commonly known as Portunus, and is an important large-scale marine economic crab in my country. Swimming crab meat is delicious and nutritious. It enjoys a high reputation at home and abroad and is deeply loved by consumers. Portunus trituberculatus adapts to a salinity of 20-35. However, because it is mainly cultured in outdoor ponds or tidal flats, the salinity of the cultured water often drops sharply due to factors such as heavy rains, which makes the feeding rate, metamorphosis rate and survival rate of young crabs Significantly decreased, it will also affect the normal molting and growth of adult crabs. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吕建建张文赵笑颜刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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