Tumor cell detection probe and preparation method and application thereof

A tumor cell and detection probe technology, which is applied in the field of tumor cell detection probes and their preparation, can solve the problems of high cost and complicated probe design, and achieves high detection sensitivity, good targeting, fluorescence imaging, and detection. Accurate and efficient results

Inactive Publication Date: 2020-02-04
南京迪安医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN105694851A discloses a fluorescent probe for tumor-targeted diagnosis and treatment. The probe uses a tumor-targeted polypeptide and an apoptotic enzyme-specific recognition polypeptide sequence as a backbone, and consists of a fluorescent pair of fluorescence quenching molecules and a photosensitizer for photodynamic therapy. Targeted therapy of tumors, and reduce the toxic and side effects of photodynamic therapy photosensitizers, while realizing photodynamic therapy of tumors, the therapeutic effect can be evaluated in situ, accurately and in real time, however, the design of the probe is relatively complex and costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this embodiment, a tumor cell detection probe is provided, and the tumor cell detection probe includes a carrier, a low molecular weight heparin-modified cadmium telluride quantum dot and a tumor targeting ligand loaded on the carrier; the carrier It is a PEG-PLA block polymer, wherein the number average molecular weight of the PEG-PLA block polymer is 5000, and the low molecular weight heparin-modified cadmium telluride quantum dots are loaded on the carrier through carboxyl-hydroxyl crosslinking, and the tumor The targeting ligand is loaded on the carrier through amino-carboxyl crosslinking; the molecular weight of the low molecular weight heparin is 3500, the tumor targeting ligand is RGD peptide, and the carrier, low molecular weight heparin modified cadmium telluride quantum dots The molar ratio with tumor targeting ligand is 20:8:1.

[0029] The tumor cell detection probe is self-assembled to form a self-assembled nanometer system.

Embodiment 2

[0031] In this embodiment, a tumor cell detection probe is provided, and the tumor cell detection probe includes a carrier, a low molecular weight heparin-modified cadmium telluride quantum dot and a tumor targeting ligand loaded on the carrier; the carrier It is a PEG-PLA block polymer, wherein the number average molecular weight of the PEG-PLA block polymer is 2000, and the low molecular weight heparin-modified cadmium telluride quantum dots are loaded on the carrier through carboxyl-hydroxyl crosslinking, and the tumor The targeting ligand is loaded on the carrier through amino-carboxyl cross-linking; the molecular weight of the low molecular weight heparin is 5500, the tumor targeting ligand is RGD peptide, and the carrier, low molecular weight heparin modified cadmium telluride quantum dots The molar ratio to the tumor targeting ligand is 42:25:1.

[0032] The tumor cell detection probe is self-assembled to form a self-assembled nanometer system.

Embodiment 3

[0034] In this embodiment, a tumor cell detection probe is provided, and the tumor cell detection probe includes a carrier, a low molecular weight heparin-modified cadmium telluride quantum dot and a tumor targeting ligand loaded on the carrier; the carrier It is a PEG-PLA block polymer, wherein the number average molecular weight of the PEG-PLA block polymer is 8000, and the low molecular weight heparin-modified cadmium telluride quantum dots are loaded on the carrier through carboxyl-hydroxyl crosslinking, and the tumor The targeting ligand is loaded on the carrier through amino-carboxyl cross-linking; the molecular weight of the low molecular weight heparin is 4000, the tumor targeting ligand is RGD peptide, and the carrier, low molecular weight heparin modified cadmium telluride quantum dots The molar ratio with tumor targeting ligand is 80:35:1.

[0035] The tumor cell detection probe is self-assembled to form a self-assembled nanometer system.

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Abstract

The invention provides a tumor cell detection probe and a preparation method and application thereof. The tumor cell detection probe comprises a carrier, low molecular weight heparin modified cadmiumtelluride quantum dots loaded on the carrier and a tumor targeting ligand, wherein the carrier is a PEG-PLA block polymer. The PEG-PLA block polymer selected by the carrier in the tumor cell detectionprobe can be self-assembled to enable the tumor cell detection probe to form a nano structure, so that the tumor cell detection probe can stably exist in a body fluid environment, the tumor can be targeted based on the targeting effect of the tumor targeting ligand, the detection and diagnosis of the tumor can be realized through quantum dot imaging at the tumor part, the detection sensitivity ishigh, and the detection is accurate and efficient.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a tumor cell detection probe and its preparation method and application. Background technique [0002] At present, in the world, the treatment methods for tumors mainly include tiger point therapy, radiotherapy, and surgical treatment. Surgical therapy is the preferred treatment method for tumor treatment. Its advantages are that it can quickly and significantly reduce tumor volume, prolong patient survival, and has no drug resistance. sexual issues. However, whether the operation can be performed in a timely and accurate manner depends on whether the tumor cells can be clearly and accurately detected to guide the operation accurately. [0003] Based on this demand, some tumor imaging technologies have been developed, and targeted tumor probes have received widespread attention, because they can specifically target tumors well, and provide help for rapid tumor locatio...

Claims

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Application Information

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IPC IPC(8): A61K49/00B82Y20/00B82Y40/00G01N21/64
CPCA61K49/0052A61K49/0054A61K49/0056A61K49/0058A61K49/0067A61K49/0093B82Y20/00B82Y40/00G01N21/6486
Inventor 冯涛陈国动周慧张楠朱红甜
Owner 南京迪安医学检验所有限公司
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