Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Disease-resistant gene collectin of lectin family of cynoglossus semilaevis

A lectin, a family of technologies, applied in the field of molecular biology, can solve problems such as undiscovered antibacterial functions

Pending Publication Date: 2020-01-31
QINGDAO UNIV +1
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the gene involved in the present invention is only considered as a simple signal recognition molecule in traditional research, and its important antibacterial function has not been found

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Disease-resistant gene collectin of lectin family of cynoglossus semilaevis
  • Disease-resistant gene collectin of lectin family of cynoglossus semilaevis
  • Disease-resistant gene collectin of lectin family of cynoglossus semilaevis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Cloning and sequence analysis of the full-length cDNA of the collagen lectin collectin 11 gene of half-smooth tongue sole

[0031] The applicant first screened out a novel lectin family gene collectin 11 with great differential expression through the association analysis (GWAS) of the transcriptome and genome of semi-smooth tongue sole injected with Vibrio anguillarum infection symptoms and those without infection symptoms. The partial mRNA sequence of collectin 11 gene was obtained by horizontal gene amplification, and then the full-length target gene was obtained by RACE-PCR technology, and the full-length cDNA sequence of the collagen lectin 11 gene in half-smooth tongue sole was obtained.

[0032] (1) The sequences of RACE primers and amplification primers used are as follows:

[0033]

[0034]

[0035](2) Experimental method: including Trizol extraction of RNA, cDNA synthesis, PCR amplification, vector construction, transfection of cells, cell cult...

Embodiment 2

[0040] Example 2: Analysis of the expression pattern of the collectin11 gene of half-smooth tongue sole in various tissues of normal fish

[0041] The qRT-PCR primers were designed according to the sequence of collectin11 gene of half-smooth tongue sole. For details of the experimental steps, see the instruction manual of the (SYBR Green) fluorescence quantitative kit, and the qRT-PCR reaction was performed on the ABI-7500Fast real-time quantitative amplification instrument. The qRT-PCR detection results were represented by the mean ± standard error (SE) of three groups of repetitions, the relative expression was calculated using the 2-ΔΔCt method, and the expression histogram was drawn using Origin9.0 ( figure 2 ).

Embodiment 3

[0042] Example 3: Relative expression analysis of the collectin11 gene of tongue sole in half-smooth tongue sole in immune-related tissues after infection with Vibrio vine

[0043] The Vibrio anguillarum infection experiment was carried out on semi-smooth tongue sole, using the Vibrio vine preserved in the laboratory, and the injection dose was 2×10 7 cfu / g, at six time points of 0h, 6h, 12h, 24h, 48h, and 72h after injection, six tissues of liver, spleen, kidney, intestine, gills and blood were collected respectively. Fluorescent quantitative PCR was used to detect the expression of collectin 11 gene in the above six tissues. The primers, reagents and experimental methods used are the same as those described in Example 2.

[0044] The results showed that the expression levels of collectin 11 in various tissues were up-regulated to varying degrees after the injection of Vibrio anguillarum: among them, in the intestine and liver, the expression of collectin 11 was always incre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a disease-resistant and bacteria-resistant related gene screened from cynoglossus semilaevis, with the gene sequence shown in SEQ ID NO: 1 and the encoded protein shown in SEQ ID NO: 2. The gene provided by the invention is related to immunity and disease resistance, the gene expression is related to the stimulation of pathogenic bacteria, and the protein expression by recombination in vitro can obviously inhibit the proliferation of gram-negative bacteria and gram-positive bacteria. The invention also provides a gene with important application and a eukaryotic expression vector pcDNA3.1-collectin containing the cynoglossus semilaevis lectin family collectin complete peptide gene and constructed by the gene; and the invention further provides a protein-producible cell line and a preparation method of the cynoglossus semilaevis lectin family collectin. Therefore, the invention has application value in the aspects of cynoglossus semilaevis disease control, feed additives, bactericides, etc.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a collectin disease-resistant gene of tongue sole family lectin. Background technique [0002] Semi-smooth tongue sole (Cynoglossus semilaevis) is rich in high-quality protein and is widely loved by consumers because of its delicious meat. In order to meet the market demand, the breeding scale and breeding density have been continuously expanded in recent years. However, with the expansion of the breeding scale and high-density breeding, the sudden occurrence of various diseases has greatly reduced the yield and quality of tongue sole. At present, the prevention and treatment of fish bacterial venereal diseases still relies heavily on antibiotics and other bactericidal drugs in the traditional technology, but the abuse of antibiotics and other drugs produces a large amount of drug residues, induces bacterial resistance, and the residual Antibiotics in tongu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/46C12N15/12C12N15/85C12N5/10A23K20/147
CPCC07K14/461A23K20/147
Inventor 沙珍霞陈亚东
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com