Acinetobacter baumannii infection fast detection method based on multiple epitope fusion antigens

Abstract

The invention relates to a preparation method of an acinetobacter baumannii infection fast detection card based on multiple epitope fusion antigens. The detection card consists of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC plate, wherein colored latex microsphere coupled anti-acinetobacter baumannii surface protein multiple epitope fusion antigen polyclonal antibodies are sprayed and coated on the combination pad; and the nitrocellulose membrane comprises detection lines coated with anti-acinetobacter baumannii surface protein multiple epitopefusion antigen polyclonal antibodies and quality control lines coated with goat anti rabbit IgG antibodies. When acinetobacter baumannii is contained in an added sample, the acinetobacter baumannii firstly forms a compound with latex-rabbit anti-acinetobacter baumannii surface protein polyclonal antibodies, and is captured when migrating to detection lines coated with the anti-acinetobacter baumannii surface protein polyclonal antibodies under the capillary action; and the detection lines show corresponding colors, so that whether the acinetobacter baumannii is contained in the sample or not can be detected. The detection card has the advantages of high speed, simplicity, high speed, high sensitivity and high specificity.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a rapid detection method for Acinetobacter baumannii infection based on multiple epitope fusion antigens. Background technique [0002] Acinetobacter baumannii (Ab) is a non-fermenting gram-negative bacillus that widely exists in nature and is an opportunistic pathogen. This bacterium is an important pathogen of nosocomial infection, mainly causing respiratory tract infection, and can also cause bacteremia, urinary tract infection, secondary meningitis, surgical site infection, ventilator-associated pneumonia, etc. The rate of resistance to commonly used antibiotics is increasing year by year, and has caused serious concern for clinicians and microbiologists. Domestic data show that A.baumannii accounts for more than 70% of clinically isolated Acinetobacter. The drug resistance rate of A.baumannii to the third and fourth generation cephalosporins has reached 63.0%-89.9%. My...

AI Technical Summary

Problems solved by technology

[0003]At present, the method for detecting the pathogen in the respiratory tract is mainly based on the traditional method, that is, the separation and identification method. Difficult to meet the needs of rapid identification; the PCR technology developed in recent years is a fast, sensitive, and specific technology, but at present this technology still relies on the pre-enrichment step of the traditional method, and the enrichment solution often There are also PCR inhibitors, which affect the effect of amplification

At the same time, this technology also requires professional testing equipment, which is not suitable for bedside testing

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