Application of segment of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular spines

Abstract

The invention belongs to the field of molecular biology, and discloses application of a segment of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular spines. The nucleotide sequence is shown as SEQ ID NO.1, by using a conventional method in the field, the SEQ ID NO.1 is taken as a target gene to perform gene mutation operation, F0 with mutation is screened out, F1 generated by hybridization of F0 and a wild type is subjected to genotyping, and mutant F1 is screened out and selfed, so that the zebra fish strain with few intramuscular spines which is remarkablyreduced by more than 70% in the number of intramuscular spines and is stably inherited can be obtained. According to the application of the segment of isolated nucleotide sequence in construction ofzebra fish with reduced intramuscular spines, the segment of isolated nucleotide sequence is used to obtain a fish strain with reduced intramuscular spines for the first time, and the application of the segment of isolated nucleotide sequence in construction of zebra fish with reduced intramuscular spines has the advantages of remarkable character improvement effect, short breeding time, stable heredity, low cost and the like, has the basic value and the application value of scientific research, can be used for researching the development mechanism of the intramuscular spines of the fish, andprovides a method for obtaining a stable genetic strain with few intramuscular spines of various fish.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to the application of an isolated nucleotide sequence in the construction of zebrafish with reduced intermuscular spines. By mutating the nucleotide sequence, zebrafish intermuscular spines can be reduced and simultaneously obtained Stably inherited zebrafish mutants with reduced intermuscular spines. Background technique [0002] The intermuscular bone is a needle-like skeleton that only exists in the muscles of lower teleosts, and is a membranous bone derived from the continuous homologous ossification of the connective tissue of the diaphragm (Patterson and Johnson, 1995; Danos and Ward ,2012). Almost half of the main farmed fishes in the world belong to Cypriniformes, and these fishes all have a certain number of intermuscular spines, such as crucian carp (Carassius carassius), grass carp (Ctenopharyngodonidella), silver carp (Hypophthalmichthys molitrix), etc. Cypr...

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Problems solved by technology

Existing studies have also explored molecular genetic information related to the development and number of intermuscular spines (Wan et al., 2016; Nie et al., 2017; Wan et al., 2017; Nie et al., 2018; Wan et al., 2019), provided the genetic data basis for the study of intermuscular spines, but did not screen out the genetic information directly related to the number of intermuscular spines

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