Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multi-crosslinking hydrogel for meniscus repair and preparation method of hydrogel

A multiple cross-linking and meniscus technology, applied in the field of medical materials, can solve the problems of different uses and differences in physical/chemical properties

Active Publication Date: 2020-01-14
JIANGSU DISSUETECH MEDICAL TECH CO LTD
View PDF12 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, on the one hand, this technical scheme needs to rely on star macromolecule or comb-like macromolecule as starting material, and this just is completely different from the present invention; On the other hand, although component a and component b in this technical scheme can Each has different active groups and these active groups have a large selection range. However, which specific active groups should be carried by the two components and what proportion / quantity of specific active groups should be carried respectively. These factors are often would result in fundamentally different physical / chemical properties of the resulting material, which would lead to very different uses, which is not addressed in the technical proposal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-crosslinking hydrogel for meniscus repair and preparation method of hydrogel
  • Multi-crosslinking hydrogel for meniscus repair and preparation method of hydrogel
  • Multi-crosslinking hydrogel for meniscus repair and preparation method of hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1, multiple cross-linked meniscus repair hydrogel complete set of raw material reagents

[0071] The multi-crosslinked meniscus repair water gel complete set of raw material reagents in this embodiment is composed of a first component and a second component, the first component is a modified collagen substance, and the modified collagen substance has a thiol-containing and the side chain of the amino group, the collagen substance is one of collagen, atelocollagen, gelatin, and collagen peptide; the second component is a modified polysaccharide, and the modified polysaccharide has a side chain containing an aldehyde group and a succinimide , the polysaccharide is one of hyaluronic acid, dextran, sodium alginate, chitin, and starch. (note: there are many types of collagen, and it has been verified by experiments that the difference of collagen types does not affect the performance of the final hydrogel of the present invention, so the collagen in each embodiment a...

Embodiment 2

[0095] Embodiment 2, multiple cross-linked meniscus repair hydrogel

[0096] The multi-crosslinked meniscus repair hydrogel of this embodiment is prepared from the multi-crosslinked meniscus repair hydrogel kit of raw material reagents in Example 1 through the following steps:

[0097] T1. Dissolve the first component in trehalose-normal saline and adjust the pH to weak acidity with hydrochloric acid to form a first solution of 5-40 mg / mL; trehalose-normal saline is physiological saline containing trehalose, wherein chlorine The concentration of sodium chloride is 9 mg / mL, and the concentration of trehalose is 20-100 mg / mL; weak acidity refers to 5.0<pH<7.0, and the pH of the first solution in this embodiment is 5.5-6.0.

[0098] Dissolve the second component in normal saline and adjust the pH to weakly alkaline with sodium hydroxide solution to form a second solution of 5-40 mg / mL; the concentration of sodium chloride in normal saline is 9 mg / mL; weakly alkaline Refers to 7....

Embodiment 3

[0107] Embodiment 3, compression modulus measurement experiment

[0108] In this example, the hydrogel of Example 3 of Example 2, comparative hydrogel 1, comparative hydrogel 2, comparative hydrogel 3, and comparative hydrogel 4 were used to measure their compressive modulus respectively.

[0109] Contrast hydrogels 1 to 4 involve 4 components altogether, and these 4 components are made by the following methods respectively:

[0110] (1) No. 1 component: thiol-modified collagen, the preparation process of which is as follows:

[0111] Dissolve 1 g of type I collagen in 100 mL of 0.2 M acetic acid to form a 10 mg / mL solution, add 1 g of EDC and 1 g of NHS in sequence, and stir and mix for 30 minutes. Then add 0.2 g of cystamine, stir and react at room temperature for 24 hours, and use hydrochloric acid and sodium hydroxide solution to adjust the pH of the system to maintain between 4 and 5 during the reaction. After 24 hours of reaction, 0.37 g of TCEP was added, and stirring...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
strengthaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention relates to a multi-crosslinking hydrogel for meniscus repair and a preparation method of the hydrogel. The hydrogel is prepared from a first solution and a second solution, a first component contained in the first solution is a modified collagen substance with a side chain containing a sulfydryl group and an amino group, and a second component contained in the second solution is modified polysaccharide with a side chain containing an aldehyde group and succinimide. The preparation method includes blending the first solution and the second solution. The multi-crosslinking meniscusrepair hydrogel can be applied to meniscus sutures to form a protective barrier, and can protect the meniscus sutures from physical wearing of articular cartilages in a recovery period; the gel is endowed with a self-repairing function through dynamic crosslinking, tiny defects of the gel can be automatically repaired, healing of sutured wounds can be promoted, tiny gaps in sutured positions arefilled, and therefore meniscus repairing is promoted, the postoperative recovery time is shortened, and the effect after healing is improved; in addition, the gel can also be used for filling mild articular surface defects, and cartilage defects are repaired in cooperation with a micro-fracture operation.

Description

technical field [0001] The invention relates to a multi-crosslinked meniscus repair hydrogel and a preparation method thereof, belonging to the technical field of medical materials. Background technique [0002] As far as the applicant knows, meniscus injury is one of the most common knee joint injuries, because the knee joint lacks blood supply, and most nutrients can only be provided through the circulation of synovial fluid. Once the meniscus is damaged, it is difficult to repair itself. The meniscus is divided into a red area and a white area. The red area is the peripheral blood supply area, and the injury in this area is easier to heal; once the injury occurs in the white area, that is, the ischemic supply area, self-healing will basically not occur. [0003] Common clinical meniscus repair techniques include arthroscopic-based inside-outside technique, outside-inside technique, all-inside technique, T-Fix technique, and meniscus arrow. Among them, the T-Fix suture t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/26A61K9/06A61K47/36A61K47/42C08H1/00
CPCA61K9/0024A61K9/06A61K47/36A61K47/42A61L27/26A61L27/52A61L2430/06A61P19/00C08H1/00C08L89/00
Inventor 周明君曹彬万文倩朱健
Owner JIANGSU DISSUETECH MEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products