Multi-crosslinking hydrogel for meniscus repair and preparation method of hydrogel
A multiple cross-linking and meniscus technology, applied in the field of medical materials, can solve the problems of different uses and differences in physical/chemical properties
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Embodiment 1
[0070] Example 1, multiple cross-linked meniscus repair hydrogel complete set of raw material reagents
[0071] The multi-crosslinked meniscus repair water gel complete set of raw material reagents in this embodiment is composed of a first component and a second component, the first component is a modified collagen substance, and the modified collagen substance has a thiol-containing and the side chain of the amino group, the collagen substance is one of collagen, atelocollagen, gelatin, and collagen peptide; the second component is a modified polysaccharide, and the modified polysaccharide has a side chain containing an aldehyde group and a succinimide , the polysaccharide is one of hyaluronic acid, dextran, sodium alginate, chitin, and starch. (note: there are many types of collagen, and it has been verified by experiments that the difference of collagen types does not affect the performance of the final hydrogel of the present invention, so the collagen in each embodiment a...
Embodiment 2
[0095] Embodiment 2, multiple cross-linked meniscus repair hydrogel
[0096] The multi-crosslinked meniscus repair hydrogel of this embodiment is prepared from the multi-crosslinked meniscus repair hydrogel kit of raw material reagents in Example 1 through the following steps:
[0097] T1. Dissolve the first component in trehalose-normal saline and adjust the pH to weak acidity with hydrochloric acid to form a first solution of 5-40 mg / mL; trehalose-normal saline is physiological saline containing trehalose, wherein chlorine The concentration of sodium chloride is 9 mg / mL, and the concentration of trehalose is 20-100 mg / mL; weak acidity refers to 5.0<pH<7.0, and the pH of the first solution in this embodiment is 5.5-6.0.
[0098] Dissolve the second component in normal saline and adjust the pH to weakly alkaline with sodium hydroxide solution to form a second solution of 5-40 mg / mL; the concentration of sodium chloride in normal saline is 9 mg / mL; weakly alkaline Refers to 7....
Embodiment 3
[0107] Embodiment 3, compression modulus measurement experiment
[0108] In this example, the hydrogel of Example 3 of Example 2, comparative hydrogel 1, comparative hydrogel 2, comparative hydrogel 3, and comparative hydrogel 4 were used to measure their compressive modulus respectively.
[0109] Contrast hydrogels 1 to 4 involve 4 components altogether, and these 4 components are made by the following methods respectively:
[0110] (1) No. 1 component: thiol-modified collagen, the preparation process of which is as follows:
[0111] Dissolve 1 g of type I collagen in 100 mL of 0.2 M acetic acid to form a 10 mg / mL solution, add 1 g of EDC and 1 g of NHS in sequence, and stir and mix for 30 minutes. Then add 0.2 g of cystamine, stir and react at room temperature for 24 hours, and use hydrochloric acid and sodium hydroxide solution to adjust the pH of the system to maintain between 4 and 5 during the reaction. After 24 hours of reaction, 0.37 g of TCEP was added, and stirring...
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