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Swine erysipelas vaccine enrichment medium

A technology for enriching culture medium and porcine erysipelas, applied in the field of biology, can solve the problems of high cost of medium synthesis, instability between batches, and low number of cultured bacteria, and achieve simple operation, stable quality, and low number of cultured bacteria high effect

Pending Publication Date: 2020-01-10
青岛高科技工业园海博生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a culture medium for swine erysipelas vaccine, which solves the technical problems of high culture medium synthesis cost, low number of cultured bacteria and instability between batches in the prior art

Method used

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  • Swine erysipelas vaccine enrichment medium
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  • Swine erysipelas vaccine enrichment medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A erysipelas vaccine enrichment medium, comprising the following raw materials in parts by weight, 5.0 g of peptone, 5.0 g of yeast extract powder, 5.0 g of beef extract powder, 5.0 g of beef liver extract powder, 3.0 g of dipotassium hydrogen phosphate, and sodium dihydrogen phosphate 5.0g, glucose 5.0g, Tween-800.5g, gelatin 2.0g, purified water to 1000ml.

[0014] The preparation method of porcine erysipelas vaccine medium, the steps are as follows:

[0015] According to the above formula, the raw materials except glucose were weighed in sequence and added to purified water, fully dissolved, adjusted to pH 7.8-8.2 with sodium hydroxide solution, and sterilized at 116°C for 30 minutes. Just before use, aseptically add 12ml of glucose solution with a final concentration of 50%, and mix well.

[0016] Preparation of the 50% glucose solution: Weigh 50 g of glucose, add purified water to dissolve, make up to 100 ml, sterilize at 116° C. for 20 minutes, and store at 2-8° ...

Embodiment 2

[0018] A culture medium for swine erysipelas vaccine, comprising the following raw materials in parts by weight, 8.0 g of peptone, 8.0 g of yeast extract powder, 8.0 g of beef extract powder, 8.0 g of beef liver extract powder, 4.0 g of dipotassium hydrogen phosphate, and sodium dihydrogen phosphate 6.0g, glucose 8.0g, Tween-800.8g, gelatin 4.0g, purified water to 1000ml.

[0019] The preparation method of porcine erysipelas vaccine medium, the steps are as follows:

[0020] According to the above formula, the raw materials except glucose were weighed in sequence and added to purified water, fully dissolved, adjusted to pH 7.8-8.2 with sodium hydroxide solution, and sterilized at 116°C for 30 minutes. Just before use, aseptically add 12ml of glucose solution with a final concentration of 50%, and mix well.

[0021] Preparation of the 50% glucose solution: Weigh 50 g of glucose, add purified water to dissolve, make up to 100 ml, sterilize at 116° C. for 20 minutes, and store a...

Embodiment 3

[0023] A erysipelas vaccine enrichment medium, comprising the following raw materials in parts by weight, 10.0 g of peptone, 10.0 g of yeast extract powder, 10.0 g of beef extract powder, 10.0 g of beef liver extract powder, 4.0 g of dipotassium hydrogen phosphate, and sodium dihydrogen phosphate 8.0g, glucose 10.0g, Tween-801.0g, gelatin 5.0g, purified water to 1000ml.

[0024] The preparation method of porcine erysipelas vaccine medium, the steps are as follows:

[0025] According to the above formula, the raw materials except glucose were weighed in sequence and added to purified water, fully dissolved, adjusted to pH 7.8-8.2 with sodium hydroxide solution, and sterilized at 116°C for 30 minutes. Just before use, aseptically add 12ml of glucose solution with a final concentration of 50%, and mix well.

[0026] Preparation of the 50% glucose solution: Weigh 50 g of glucose, add purified water to dissolve, make up to 100 ml, sterilize at 116° C. for 20 minutes, and store at ...

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Abstract

The invention relates to a swine erysipelas vaccine enrichment medium, which relates to the field of biology. The swine erysipelas vaccine enrichment medium comprises the following raw materials in parts by weight: 5.0-10.0g of peptone, 5.0-10.0g of yeast extract, 5.0-10.0g of beef extract, 5.0-10.0g of beef liver extract, 3.0-5.0g of dipotassium phosphate, 5.0-8.0g of sodium dihydrogen phosphate,5.0-1.0g of glucose, 50-1.0g of Tween 80, 2.0-5.0g of gelatin, and purified water added to 1000ml; a preparation method of swine erysipelas vaccine culture medium comprises the following steps: all raw materials except glucose for the preparation of swine erysipelas vaccine medium are added to purified water, and after being fully dissolved, a sodium hydroxide solution is used to adjust a pH value to 7.8-8.2 and sterilizing is carried out at 116 DEG C for 30 minutes, 12ml of a glucose solution with the final concentration of 50% is added by sterile operation before use, and the materials areuniformly mixed; the preparation of the 50% glucose solution comprises the following steps: weighing 50g of glucose, adding purified water for dissolving, fixing the volume to 100ml, sterilizing at 116 DEG C for 20 minutes, and storing at 2-8 DEG C. The invention relates to an application of a synthetic dry powder culture medium in production of swine erysipelas vaccines. The synthetic dry powderculture medium has the beneficial effects of suitability for high-density culture of swine erysipelas vaccine thalli, selection of commercial raw materials and high bacterial count of cultured bacteria.

Description

technical field [0001] The invention relates to the technical field of biology, in particular to a culture medium for enriching porcine erysipelas vaccine. Background technique [0002] Erysipelas is one of the three major infectious diseases of pigs. It is distributed worldwide and is also a zoonotic disease. The source of the disease is Erysipelas suis. At present, the live vaccines for porcine erysipelas in my country include single vaccine, double vaccine and triple vaccine, which play an important role in preventing porcine erysipelas disease. However, there is still a large gap with developed countries in terms of vaccine culture. First, the culture stability is poor. In the 1960s and 1970s, the meat liver stomach (membrane) digestion soup containing 2%-4% lysed whole blood was used as the medium for the production of vaccines. Since the 1980s, domestic veterinary biological products companies have begun to use Tween-80 instead of lysed blood cells. The production o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 蔡向荣许英俊
Owner 青岛高科技工业园海博生物技术有限公司
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