Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cold plasma-treated twin-arginine antibacterial agent and preparation method and application thereof

A technology of cold plasma and double arginine, which is applied in the field of double arginine antibacterial agent and preparation, to achieve low dose and improve antibacterial effect

Pending Publication Date: 2020-01-03
JIANGSU UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no patent on double arginine treated with cold plasma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cold plasma-treated twin-arginine antibacterial agent and preparation method and application thereof
  • Cold plasma-treated twin-arginine antibacterial agent and preparation method and application thereof
  • Cold plasma-treated twin-arginine antibacterial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Antibacterial effect of double arginine antibacterial agent treated with cold plasma against Salmonella

[0022] 1 Experimental materials

[0023]

[0024] 2 Experimental methods

[0025] 1) Preparation of plasma-treated double arginine

[0026] Place the double arginine solid powder in a plasma treatment apparatus and treat it with 300-500W cold plasma for 3-5 minutes. Untreated double arginine solid powder and plasma treated double arginine solid powder were respectively added to 3% acetic acid solution to obtain a concentration of 9 mg / mL, and were fully dissolved under magnetic stirring at a speed of 25 r / min for 30 min. The antibacterial agent is obtained.

[0027] 2) Antibacterial effect in PBS

[0028] ① Salmonella was inoculated in nutrient broth liquid medium, and cultured at 37°C with shaking (150rpm) for 24 hours to obtain Salmonella in logarithmic growth phase.

[0029] ②Add the Salmonella in the logarithmic growth phase to the sterile phosphate buff...

Embodiment 2

[0042] Antibacterial effect of double arginine antibacterial agent treated with cold plasma against methicillin-resistant Staphylococcus aureus (MRSA)

[0043] 1 Experimental materials

[0044]

[0045] 2 Experimental methods

[0046] 1) Preparation of plasma-treated double arginine

[0047] Place the double arginine solid powder in a plasma treatment apparatus and treat it with 300-500W cold plasma for 3-5 minutes. Untreated double arginine solid powder and plasma treated double arginine solid powder were respectively added to 3% acetic acid solution to obtain a concentration of 9 mg / mL, and were fully dissolved under magnetic stirring at a speed of 25 r / min for 30 min. The antibacterial agent is obtained.

[0048] 2) Antibacterial effect in PBS

[0049] ①Methicillin-resistant Staphylococcus aureus was inoculated in nutrient broth liquid medium, and cultured at 37°C with shaking (150rpm) for 48 hours to obtain methicillin-resistant Staphylococcus aureus in logarithmic ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of food safety, and particularly relates to a cold plasma-treated twin-arginine antibacterial agent and a preparation method and application thereof. The excellent antibacterial effect of twin-arginine is utilized, the surface of a twin-arginine powder is modified through cold plasmas, the antibacterial effect is further improved, and the safe and environment-friendly novel antibacterial agent free from toxic or side effect is prepared. The novel antibacterial agent can effectively kill various food-borne pathogenic bacteria, and nosocomial bacteria such as methicillin-resistant staphylococcus aureus. Compared with untreated twin-arginine, the twin-arginine treated by the cold plasmas in a phosphate buffer solution and broth has obviously-improved antibacterial effect.

Description

technical field [0001] The invention belongs to the field of food safety, and in particular relates to a cold plasma-treated double arginine antibacterial agent, a preparation method and an application. Background technique [0002] There are many kinds of existing antibacterial agents, most of which use organic antibacterial agents with certain toxicity, such as vanillin or ethyl vanillin compounds. In this context, there is an urgent need for a safe, non-toxic, and broad-spectrum antibacterial antibacterial agent in the field of food safety. [0003] Double arginine is one of the smallest biological self-assembly materials. Its structural formula is as follows. It is formed by dehydration condensation of two arginines, and one end is amino-NH 2 , one end is carboxyl-COOH, which exists in the form of amphoteric ions at the isoelectric point, that is, one end is -NH 3 + , one end is -COO - . Double arginine can self-assemble in many solvents, such as methanol, chlorofor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3526A23L5/30
CPCA23L3/3526A23L5/30
Inventor 林琳廖雪崔海英朱玉林
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com