A kind of human dip-indel loci fluorescent labeling kit and detection method
A fluorescent labeling and dip-indel technology, which is applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as high mutation rate, fetal death, intrauterine infection, etc., and achieve the goal of protecting safety and improving accuracy Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] Embodiment 1: Population frequency survey.
[0053] 1. Collect individual test materials.
[0054] A total of 200 unrelated individual samples were collected in this experiment, all of which belonged to the Han population in Shanxi. Genomic DNA was extracted according to "GA / T 383-2014 Forensic Science DNA Laboratory Inspection Specification".
[0055] 2. Amplification and detection.
[0056] Using the extracted DNA as a template, PCR amplification was carried out. The PCR reaction system is (10 μL): 5 μL 2×PCRMasterMix, add different volumes of primers according to Table 2, 10ng template DNA, ddH 2 O to make up to 10 μL.
[0057] The amplification program was: 95°C for 10 min; 95°C for 30 s, 56°C for 30 s, 72°C for 30 s, 5 cycles; 94°C for 30 s, 52°C for 30 s, 68°C for 30 s, 25 cycles; 68°C for 30 min.
[0058] Detection of amplification products: 1.5 μL of amplification products, 10 μL of deionized formamide, and 0.2 μL of SIZE-5000Plus. Denaturation method: aft...
Embodiment 2
[0061] Example 2: Study on species specificity.
[0062] 1. Collect animal samples.
[0063] The animal and bacterial DNA samples in this experiment were from cattle, fish, pigs, rabbits, mice, sheep and streptococci, respectively. Genomic DNA was extracted using the Qiagen (DNeasy Blood & Tissue Kit) kit.
[0064] 2. Amplification and detection.
[0065] Carry out PCR amplification, detection and result analysis according to the method in Example 1, the result proves that this kit has species specificity.
Embodiment 3
[0066] Embodiment 3: Sensitivity detection.
[0067] 1. Prepare DNA samples.
[0068] DNA was extracted using the Qiagen (DNeasy Blood & Tissue Kit) kit. The sensitivity of the system was determined using single-source DNA containing different amounts of DNA template (6ng, 4ng, 2ng and 1ng).
[0069] 2. Amplification and detection.
[0070] PCR amplification, detection and result analysis were carried out according to the method in Example 1. image 3 It is confirmed that the sensitivity of this kit is good, and the minimum detectable content is as low as 2ng of DNA.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com