High-clarity soybean flavor peptide, preparation method and use thereof
A soybean and soybean protein technology, applied in the field of high-clarity soybean flavor peptide and its preparation, can solve the problems of reducing bioavailability and nutrition, affecting food appearance, limiting soybean flavor peptide, etc., and achieving the promotion of insoluble matter The effect of simple formation, process operation and high production efficiency
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Embodiment 1
[0040] A preparation method of high-clarity soybean flavor peptide, comprising the following steps:
[0041] (1) Mix 1 part of soybean meal with 3 parts of water, raise the temperature to 50°C, heat and stir for 3.0 hours, and then pass through a colloid mill to obtain a soybean protein slurry;
[0042] (2) Add 1.2% papain and 0.4% flavor protease and 0.5% cellulase to the soybean protein slurry, heat and hydrolyze it at 50°C, and when the degree of hydrolysis reaches 15%, pass through the filter Cloth is a 400-mesh filter for separation, and the filtrate is collected to obtain the soybean enzymatic hydrolysis liquid; the soybean enzymatic hydrolysis liquid is centrifuged with a three-phase disc separator, and the upper oil layer and the bottom slag are discarded to obtain the intermediate clear liquid as soybean Taste peptide liquid;
[0043] (3) Use food-grade citric acid to adjust the pH value of the soybean flavor peptide liquid to 4.5, and then concentrate under reduced ...
Embodiment 2
[0048] A preparation method of high-clarity soybean flavor peptide, comprising the following steps:
[0049] (1) Mix 1 part of soybean meal with 5 parts of water, raise the temperature to 65°C, heat and stir for 2.0 hours, and then pass colloid milling to obtain soybean protein slurry;
[0050] (2) Add 0.6% papain and 0.2% flavor protease to soybean protein slurry, and 0.8% cellulase, hydrolyze at 60°C, when the degree of hydrolysis reaches 10%, pass through the filter cloth Separation with a 600-mesh filter, collecting the filtrate to obtain soybean enzymatic hydrolysis liquid; using a three-phase disc separator to centrifuge the soybean enzymatic hydrolysis liquid, discarding the upper oil layer and the bottom slag, and obtaining the intermediate clear liquid as soybean flavor Peptide solution;
[0051] (3) Adjust the pH value of the soybean flavor peptide liquid to 4.2 with hydrochloric acid solution, and then carry out reduced pressure concentration at a temperature of 55...
Embodiment 3
[0056] A preparation method of high-clarity soybean flavor peptide, comprising the following steps:
[0057] (1) Mix 1 part of soybeans with 4 parts of water, heat up to 55°C, heat and stir for 2.5 hours, and then pass colloid milling to obtain soybean protein slurry;
[0058] (2) Add papain and 0.2% flavor protease and 0.7% cellulase to the soybean protein slurry, hydrolyze at 55°C, when the degree of hydrolysis reaches 12%, pass through the filter cloth Separation with a 500-mesh filter, collecting the filtrate to obtain soybean enzymatic hydrolysis liquid; using a three-phase disc separator to centrifuge the soybean enzymatic hydrolysis liquid, discarding the upper oil layer and bottom slag, and obtaining the intermediate clear liquid as soybean flavor Peptide solution;
[0059] (3) Use citric acid to adjust the pH value of the soybean flavor peptide liquid to 4.4, and then concentrate under reduced pressure at a temperature of 60 ° C. When the solid content of the soybean...
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