Ion pair dual water phase method for extracting and separating pyrroloquinoline quinone
A pyrroloquinoline quinone and two-phase technology, which is applied in the field of ion-pair aqueous two-phase extraction and separation of pyrroloquinoline quinone, can solve the problems of restricting the large-scale production and application of PQQ, low extraction efficiency, poor selectivity, etc. Industrial production, simple process operation, and the effect of improving the recovery rate
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Embodiment 1
[0039] The method of ion-pair aqueous two-phase extraction and separation of pyrroloquinoline quinone in this embodiment includes the following steps:
[0040] (1) Preparation: Gluconobacter oxydans was inoculated into the fermentation medium according to the inoculation amount of 5%, and the seed solution was obtained by shaking culture at 28°C for 3 days; then the seed solution was inoculated into the fermentation medium according to the inoculation amount of 10%. Enrich the medium, culture with shaking at 28°C for 5 days, centrifuge the culture solution at 5°C, 9000r / min for 15min, collect the supernatant, and obtain the fermentation broth containing pyrroloquinoline quinone;
[0041] (2) Extraction: The ion-pair two-phase extraction system is used to extract the fermentation broth containing pyrroloquinoline quinone. The volume ratio of the extraction system and the fermentation broth containing pyrroloquinoline quinone is 4:2. The upper layer is separated and the lower layer is...
Embodiment 2
[0045] The method of ion-pair aqueous two-phase extraction and separation of pyrroloquinoline quinone in this embodiment includes the following steps:
[0046] (1) Preparation: Gluconobacter oxydans was inoculated into the fermentation medium according to the inoculum of 5%, cultured with shaking at 28°C for 3 days to obtain the seed liquid; then the seed liquid was inoculated with the inoculum of 10% Enrich the medium, culture with shaking at 28°C for 5 days, centrifuge the culture solution at 5°C, 9000r / min for 15min, collect the supernatant, and obtain the fermentation broth containing pyrroloquinoline quinone;
[0047] (2) Extraction: The ion-pair two-phase extraction system is used to extract the fermentation broth containing pyrroloquinoline quinone. The volume ratio of the extraction system and the fermentation broth containing pyrroloquinoline quinone is 5:3. The upper layer is separated and the lower layer is separated. The extraction was repeated 3 times, and the separate...
Embodiment 3
[0051] The method of ion-pair aqueous two-phase extraction and separation of pyrroloquinoline quinone in this embodiment includes the following steps:
[0052] (1) Preparation: Gluconobacter oxydans was inoculated into the fermentation medium according to the inoculation amount of 5%, and the seed solution was obtained by shaking culture at 28°C for 3 days; then the seed solution was inoculated into the fermentation medium according to the inoculation amount of 10%. Enrich the medium, culture with shaking at 28°C for 5 days, centrifuge the culture solution at 5°C, 9000r / min for 15min, collect the supernatant, and obtain the fermentation broth containing pyrroloquinoline quinone;
[0053] (2) Extraction: The ion-pair aqueous two-phase extraction system is used to extract the fermentation broth containing pyrroloquinoline quinone. The volume ratio of the extraction system and the fermentation broth containing pyrroloquinoline quinone is 7:4. The upper layer is separated and the lower ...
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