Method for extracting superoxide dismutase from plants as raw material
A technology of superoxide and plant raw materials, applied in the biological field, can solve the problems of complicated separation and extraction process, repeated addition and repeated separation, chemical pollution, etc., and achieves the effect of long activity retention time, good quality and high activity.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0025] The method for extracting superoxide dismutase from plants, the steps are as follows:
[0026] (1) Mix and pulverize golden okra, pine needles and cypress herb until the diameter is 100 meshes, then add an active protective agent of 0.02 times the mass of the mixture of golden okra, pine needles and cypress herb to carry out wall-breaking treatment to obtain a plant slurry for later use;
[0027] (2) Keep the plant slurry at a constant temperature of 50°C for 30 minutes to carry out active proliferation and fission;
[0028] (3) Centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 4000rpm, the centrifugation time is 30min, and the active liquid is obtained after removing the precipitate;
[0029] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 97 to the mass ratio of ethanol and equal mass zinc gluconate and alkaline protease mixture...
Embodiment 2
[0032] The method for extracting superoxide dismutase from plants, the steps are as follows:
[0033] (1) after the golden okra is crushed to a diameter of 80 meshes, an active protective agent of 0.005 times the quality of the golden okra is added for wall-breaking treatment to obtain a plant slurry for subsequent use;
[0034] (2) Keep the plant slurry at a constant temperature of 50°C for 25 minutes to carry out active proliferation and fission;
[0035] (3) Centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 4000rpm, the centrifugation time is 10min, and the active liquid is obtained after removing the precipitate;
[0036] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 95 to the mass ratio of ethanol and equal mass zinc gluconate and alkaline protease mixture with 60 times of zinc gluconate and alkaline protease mixture is 7: 3. Obt...
Embodiment 3
[0039] Vegetable ingredients are golden okra, sea buckthorn, pine needles, clover herb
[0040] The method for extracting superoxide dismutase from plants, the steps are as follows:
[0041] (1) Grinding golden okra and seabuckthorn to a diameter of 100 meshes, adding an active protective agent of 0.02 times the mass of the mixture of golden okra and seabuckthorn to carry out wall-breaking treatment to obtain a plant slurry for subsequent use;
[0042] (2) Keep the plant slurry at a constant temperature of 55°C for 35 minutes to carry out active proliferation and fission;
[0043] (3) centrifuging the plant slurry after active proliferation and fission, the centrifugal speed is 6000rpm, the centrifugation time is 30min, and the active liquid is obtained after removing the precipitate;
[0044] (4) Get equal amount of zinc gluconate and alkaline protease to mix, add concentration to wherein after adding the ethanol that concentration is 97 to the mass ratio of ethanol and equa...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com