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Kit for predicting hormone receptor positive breast cancer preoperative chemosensitivity

A technology sensitive to hormone receptors and chemotherapy, applied in the field of biomedicine, can solve problems such as non-recommended use, pCR rate reflection, stratified analysis, etc., and achieve the effect of clinical operation convenience

Pending Publication Date: 2019-12-24
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In 2005, Gianni et al. (Gianni et al. J Clin Oncol, 2005) performed a 21-gene recurrence risk score on 89 locally advanced NAC patients who received an anthracycline / taxane-based regimen, and planned to explore the role of the 21-gene recurrence risk score in predicting The application value of pCR after NAC, although the research results showed that the risk score was positively correlated with the pCR rate (P=0.005), but there were many limitations in the study: 89 patients included all molecular types of breast cancer, but The pCR rate was not stratified according to the molecular subtype; at the same time, the AUC value of the 21-gene recurrence risk score for predicting pCR was not clearly given; the specific pCR rate of patients with different risk scores was not reflected in the research results
Therefore, the 21 genes that are widely used clinically to predict the prognosis of HR-positive patients are not clear in predicting the curative effect in NAC, and cannot be recommended in clinical practice.

Method used

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  • Kit for predicting hormone receptor positive breast cancer preoperative chemosensitivity
  • Kit for predicting hormone receptor positive breast cancer preoperative chemosensitivity
  • Kit for predicting hormone receptor positive breast cancer preoperative chemosensitivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Extraction of total RNA

[0033] (1) Removal of paraffin

[0034] Add 2ml xylene (self-prepared) to a 5ml centrifuge tube containing paraffin tissue, vortex for 10 seconds; transfer the tissue and xylene mixture to a 2ml centrifuge tube (AXYGEN MCT-200-C), centrifuge at 12000rpm for 2min at room temperature, Gently pour off the supernatant, be careful not to remove the precipitate; add 1.5ml of xylene again, vortex for 10 seconds; centrifuge at 12000rpm at room temperature for 2min, gently pour off the supernatant, be careful not to remove the precipitate; add 1ml of absolute ethanol (prepared by yourself), Vortex and mix well, and centrifuge at 12,000rpm for 2 minutes at room temperature; pour off the supernatant gently, and be careful not to remove the precipitate. If white paraffin remains on the upper layer of the precipitate, add 1.5ml xylene again, vortex for 10 seconds, centrifuge to remove the supernatant, do not remove Precipitate; add 1ml of absolute ethan...

Embodiment 2

[0069] According to the operation method of Example 1, the pre-NAC baseline tumor puncture paraffin specimen was used to calculate the risk score (risk score, RS) of each HR-positive early breast cancer sensitive to preoperative chemotherapy. The RS distribution of 285 patients is as follows: figure 2 As shown, the ordinate of the distribution graph is the RS score, and the abscissa represents the number of patients. The specific RS scores of each enrolled patient are arranged from left to right in ascending order, and the median value of RS is 2.062 (range: - 3.753 to 7.781). figure 2 The black dots represent non-pCR patients, and the gray dots represent pCR patients. figure 2 It can be seen that the density of gray dots is densely distributed in places with high RS scores, suggesting that the higher the RS score, the more sensitive chemotherapy is, and the easier it is to obtain pCR.

[0070] Using the continuous variable 10-miRNA RS as a predictor, the logistic model wa...

Embodiment 3

[0073] In order to facilitate the qualitative assessment of the chemotherapy sensitivity of patients with pseudo-NAC in clinical practice, we further select an optimal cut-off value (cut-off) for the 10-miRNA RS model, and transform the continuous RS into a binary variable, which is more helpful Clinicians make treatment decisions. In order to select the optimal cut-off value, we used the grid search method, and performed chi-square test, Fisher's exact test and logistic regression analysis on RS with different cut-off values ​​through R language programming, and observed each possible RS value as the cut-off value prominence at the time. Specific results such as Figure 4 It shows that the abscissa is the specific RS cut-off value, the ordinate is the P value, and the dotted line is the reference line with the P value less than 0.05. The machine performs calculations in the range of RS equal to 0 to 5, and the RS value below the dotted line is used as the cut-off value , th...

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Abstract

The invention discloses an application of miRNA specific oligonucleotides in preparation of a kit for predicting hormone receptor positive breast cancer preoperative chemosensitivity. The kit is usedfor obtaining a risk score by detecting the expression level of at least one miRNA gene from a subject sample and combining a gene risk stratification calculation formula, whether the subject has therisk of hormone receptor positive breast cancer preoperative chemosensitivity or not is evaluated, a truncation value is selected as a judgment basis, the subject is at the high risk of preoperative chemosensitivity when the value is higher than the truncation value, and the subject is at the low risk of preoperative chemosensitivity when the value is lower than the truncation value; wherein the at least one miRNA gene is miR-200c. The invention further discloses a preparation method of the miRNA gene. According to the invention, the HR positive breast cancer preoperative chemosensitivity score is obtained by detecting the relative expression quantity of miRNA related to breast cancer stem cells according to a risk scoring model, so as to evaluate whether a patient is suitable for preoperative chemotherapy or not.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a kit for predicting preoperative chemotherapy sensitivity of hormone receptor positive breast cancer. Background technique [0002] Breast cancer is the most common malignancy in women, accounting for 30% of all newly diagnosed malignancies in women and 15% of cancer-related deaths. According to GLOBOCAN-2018 global cancer statistics, there were about 2 million newly diagnosed breast cancer patients worldwide in 2018, and more than 600,000 deaths. According to the 2014 cancer report in China, there were about 278,900 new cases of breast cancer in the country, and the overall incidence rate was 28.2 / 100,000. 16.51% of the incidence, ranking first. Breast cancer is a group of malignant tumors with high heterogeneity. Among them, hormone receptor (HR) positive is the most common type of breast cancer, accounting for 55%-70% of the overall proportion of breast can...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/106C12Q2600/178C12Q2600/166C12Q2600/118
Inventor 宋尔卫龚畅陈润生谭璐媛
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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