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Method for immobilizing bio-enzyme in ceramic filling material

A technology of ceramic fillers and biological enzymes, applied in the direction of fixing on or in inorganic carriers, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as easy failure, non-reusable use, and unsuitable for industrial production. achieve high activity

Inactive Publication Date: 2015-12-23
HANGZHOU CHENGJIE ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be used repeatedly and is prone to failure, so it is not suitable for industrial production

Method used

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  • Method for immobilizing bio-enzyme in ceramic filling material
  • Method for immobilizing bio-enzyme in ceramic filling material
  • Method for immobilizing bio-enzyme in ceramic filling material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of alkaline protein modified enzyme solution

[0040] Take 1 liter of distilled water, adjust the pH to 8.3 with 0.5M NaOH, add 2 ml of 30% hydrogen peroxide, and heat the water to 35°C. Weigh 200g of biological enzymes and disperse them in the above aqueous solution, vibrate with a frequency of 50K Hz ultrasonic wave for 20 minutes, then centrifuge at 5000r / min for 15 minutes, take out 600ml of supernatant, and add 2ml of 0.2mol / L borate buffer solution with a pH of 9.0 Dissolve the precipitate and filter out the insoluble matter to obtain 500ml of alkaline protein modified enzyme solution.

[0041] Modifier preparation

[0042] By weight percentage: 5% glucosamine, 10% corn steep liquor, 5% benzoyl peroxide, 1% ferric sulfate, 20% peptone, 59% ethyl acetate are mixed for later use.

[0043] Ceramic filler immobilized biological enzyme

[0044] The diameter of the particles that have been vibrated and screened to remove dust and dust is 10mm, and the spe...

Embodiment 2

[0057] Preparation of alkaline protein modified enzyme solution

[0058] Take 1 liter of distilled water, adjust the pH to 8.5 with 0.5M NaOH, add 2 ml of 30% hydrogen peroxide, and heat the water to 30°C. Weigh 200g of biological enzyme and disperse in the above aqueous solution, vibrate with frequency 40K Hz ultrasound for 20 minutes, then centrifuge at 5000r / min for 15 minutes, take out 600ml of supernatant, add 2ml of 0.2mol / L borate buffer solution with pH 9.0 Dissolve the precipitate and filter out the insoluble matter to obtain 500ml of alkaline protein modified enzyme solution.

[0059] Modifier preparation

[0060] By weight percentage: 3% glucosamine, 12% corn steep liquor, 4% benzoyl peroxide, 2% ferric sulfate, 18% peptone, 61% ethyl acetate are mixed for later use.

[0061] Ceramic filler immobilized biological enzyme

[0062] The diameter of the particles that have been vibrated and screened to remove dust and dust is 10mm, and the specific surface area is 300...

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Abstract

The invention relates to a bio-enzyme immobilization method in the field of sewage treatment technology, specifically to a method for immobilizing bio-enzyme in a ceramic filling material. The method comprises the following steps: step 1, treatment of the bio-enzyme; step 2, treatment of the ceramic filling material; and step 3, mixing of the treated bio-enzyme and ceramic filling material so as to prepare a biological ceramic filling material. Through immobilization of the enzyme on the ceramic filling material, the effect of enzymatic reaction can be improved; so various pollutants in sewage is better removed, metabolites secreted by microbes are effectively decomposed, accumulation of sludge among and in the filling material is eliminated, unique permanent high efficiency of the enzymatic reaction is given to play, and the filling material is endowed with self-purifying and self-restoration capabilities and has prolonged service life.

Description

technical field [0001] The invention relates to a method for immobilizing biological enzymes in the field of sewage treatment technology, in particular to a method for immobilizing biological enzymes in ceramic fillers. Background technique [0002] The biological aerated filter process is a new type of sewage treatment technology created in the late 1980s. Due to its characteristics of large hydraulic load, short hydraulic retention time, good effluent quality, and low operating costs, it has received more and more attention. Much attention. The essence of this process is to fill the biological reaction tank with fillers, and the surface and interior of the fillers are covered with biofilms, and the organic pollutants in the sewage are removed through the oxidation and decomposition of microorganisms in the biofilms. The speed and quality of formation, shedding and renewal will directly affect the purification effect. Compared with other microbial treatment methods, the a...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34
Inventor 童少平魏状洪夏萍朱显跃
Owner HANGZHOU CHENGJIE ENVIRONMENTAL PROTECTION
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